Establishment of a sepsis model following implantation of Klebsiella pneumoniae-infected fibrin clot into the peritoneal cavity of mice
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
14976726
DOI
10.1007/bf02993476
Knihovny.cz E-zdroje
- MeSH
- fibrin MeSH
- inbrední kmeny myší * MeSH
- infekce bakteriemi rodu Klebsiella mikrobiologie mortalita patologie MeSH
- játra mikrobiologie patologie MeSH
- Klebsiella pneumoniae * MeSH
- modely nemocí na zvířatech * MeSH
- myši MeSH
- peritoneální dutina mikrobiologie MeSH
- sepse mikrobiologie mortalita patologie MeSH
- slezina mikrobiologie patologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fibrin MeSH
Successful establishment of sepsis by entrapping a dose of 150 colony forming units of Klebsiella pneumoniae in a fibrin clot following implantation into the peritoneal cavity of mice is reported. The dose in the fibrin clot gave 50% mortality in mice, spread over a period of one week. All the infected mice showed positive blood culture up to 6 d post-infection; histopathology revealed inflammatory changes in both liver and spleen. Introduction of K. pneumoniae into experimental mice without entrapment in fibrin clot caused no mortality and blood culture remained positive only up to 2 d; histopathology of liver and spleen throughout the period of study showed relatively mild inflammatory changes, which almost cleared during 14 d post-infection. The use of the fibrin-clot model may thus be considered to be useful in studying both the initial and the persisting stage of infection in the peritoneum, whence a slow release of bacteria into the blood takes place which finally leads to sepsis and septicemia.
Zobrazit více v PubMed
J Clin Invest. 1989 Jan;83(1):243-51 PubMed
J Surg Res. 1980 Aug;29(2):189-201 PubMed
Ann Surg. 1984 Nov;200(5):619-26 PubMed
J Infect Dis. 1995 Dec;172(6):1519-27 PubMed
J Med Microbiol. 1998 Jul;47(7):591-8 PubMed
Folia Microbiol (Praha). 2001;46(5):459-62 PubMed
Ann Surg. 1989 Mar;209(3):334-40 PubMed
J Infect Dis. 1996 Jan;173(1):203-11 PubMed
Mamm Genome. 2001 Sep;12(9):673-7 PubMed
Antonie Van Leeuwenhoek. 1990 Aug;58(2):87-93 PubMed
J Med Microbiol. 2000 May;49(5):431-9 PubMed
Folia Microbiol (Praha). 2002;47(5):565-71 PubMed
J Toxicol Environ Health. 1997 Aug 8;51(5):415-35 PubMed
Science. 2002 May 31;296(5573):1661-71 PubMed
Antimicrob Agents Chemother. 1994 Jan;38(1):147-50 PubMed
