Establishment of a sepsis model following implantation of Klebsiella pneumoniae-infected fibrin clot into the peritoneal cavity of mice
Language English Country United States Media print
Document type Journal Article
PubMed
14976726
DOI
10.1007/bf02993476
Knihovny.cz E-resources
- MeSH
- Fibrin MeSH
- Mice, Inbred Strains * MeSH
- Klebsiella Infections microbiology mortality pathology MeSH
- Liver microbiology pathology MeSH
- Klebsiella pneumoniae * MeSH
- Disease Models, Animal * MeSH
- Mice MeSH
- Peritoneal Cavity microbiology MeSH
- Sepsis microbiology mortality pathology MeSH
- Spleen microbiology pathology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fibrin MeSH
Successful establishment of sepsis by entrapping a dose of 150 colony forming units of Klebsiella pneumoniae in a fibrin clot following implantation into the peritoneal cavity of mice is reported. The dose in the fibrin clot gave 50% mortality in mice, spread over a period of one week. All the infected mice showed positive blood culture up to 6 d post-infection; histopathology revealed inflammatory changes in both liver and spleen. Introduction of K. pneumoniae into experimental mice without entrapment in fibrin clot caused no mortality and blood culture remained positive only up to 2 d; histopathology of liver and spleen throughout the period of study showed relatively mild inflammatory changes, which almost cleared during 14 d post-infection. The use of the fibrin-clot model may thus be considered to be useful in studying both the initial and the persisting stage of infection in the peritoneum, whence a slow release of bacteria into the blood takes place which finally leads to sepsis and septicemia.
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