Constitutive alpha-amylase producing mutant and recombinant haploid strains of thermophilic fungus Thermomyces lanuginosus
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16110918
DOI
10.1007/bf02931462
Knihovny.cz E-zdroje
- MeSH
- alfa-amylasy biosyntéza MeSH
- Ascomycota enzymologie genetika růst a vývoj MeSH
- biotechnologie metody MeSH
- haploidie * MeSH
- morfogeneze MeSH
- mutace * MeSH
- mutageneze MeSH
- rekombinace genetická * MeSH
- spory hub MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alfa-amylasy MeSH
Morphological, developmental and antimetabolite-resistant mutants of T. lanuginosus were characterized and used for screening with the aim to develop constitutive alpha-amylase-hyperproducing strains. The protoplast fusion of two spontaneous mutants of T. lanuginosus, characterized as asporulating and resistant to 2-deoxy-D-glucose (2DG), resulted in sporulating, 2DG sensitive heterokaryotic fusants. A recombinant haploid strain F64fB developed there from produced alpha-amylase constitutively in glucose-containing medium. Constitutive alpha-amylase-hyperproducing mutant (III8) obtained after cyclic mutagenesis and screening yielded approximately 20 fold more alpha-amylase in a glycerol-containing medium than the wild strain.
Zobrazit více v PubMed
Folia Microbiol (Praha). 2003;48(4):469-78 PubMed
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6226-30 PubMed
Genes Dev. 1991 Dec;5(12B):2420-30 PubMed
J Ind Microbiol Biotechnol. 2002 Aug;29(2):70-4 PubMed
Can J Microbiol. 2000 Jul;46(7):669-73 PubMed
Folia Microbiol (Praha). 2003;48(5):597-604 PubMed
Fungal Genet Biol. 1996 Sep;20(3):193-203 PubMed
Microbiol Rev. 1986 Jun;50(2):95-132 PubMed
Appl Environ Microbiol. 1998 Nov;64(11):4423-7 PubMed
J Gen Microbiol. 1991 Aug;137(8):2017-23 PubMed
