Constitutive alpha-amylase producing mutant and recombinant haploid strains of thermophilic fungus Thermomyces lanuginosus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16110918
DOI
10.1007/bf02931462
Knihovny.cz E-resources
- MeSH
- alpha-Amylases biosynthesis MeSH
- Ascomycota enzymology genetics growth & development MeSH
- Biotechnology methods MeSH
- Haploidy * MeSH
- Morphogenesis MeSH
- Mutation * MeSH
- Mutagenesis MeSH
- Recombination, Genetic * MeSH
- Spores, Fungal MeSH
- Hot Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-Amylases MeSH
Morphological, developmental and antimetabolite-resistant mutants of T. lanuginosus were characterized and used for screening with the aim to develop constitutive alpha-amylase-hyperproducing strains. The protoplast fusion of two spontaneous mutants of T. lanuginosus, characterized as asporulating and resistant to 2-deoxy-D-glucose (2DG), resulted in sporulating, 2DG sensitive heterokaryotic fusants. A recombinant haploid strain F64fB developed there from produced alpha-amylase constitutively in glucose-containing medium. Constitutive alpha-amylase-hyperproducing mutant (III8) obtained after cyclic mutagenesis and screening yielded approximately 20 fold more alpha-amylase in a glycerol-containing medium than the wild strain.
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