Effect of procathepsin D activation peptide on gene expression of breast cancer cells
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
PubMed
16168559
DOI
10.1016/j.canlet.2005.07.030
PII: S0304-3835(05)00731-7
Knihovny.cz E-resources
- MeSH
- Cathepsin D pharmacology MeSH
- Humans MeSH
- Tumor Cells, Cultured MeSH
- Neoplasm Proteins genetics MeSH
- Breast Neoplasms genetics MeSH
- NF-kappa B p52 Subunit genetics metabolism MeSH
- Peptide Fragments pharmacology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Enzyme Precursors pharmacology MeSH
- Gene Expression Regulation, Neoplastic drug effects MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Signal Transduction MeSH
- Gene Expression Profiling MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- Cathepsin D MeSH
- Neoplasm Proteins MeSH
- NF-kappa B p52 Subunit MeSH
- Peptide Fragments MeSH
- Enzyme Precursors MeSH
- procathepsin D MeSH Browser
Overexpression of procathepsin D (pCD) is reported to occur in numerous types of cancer and is associated with increased growth and metastasis. It has been established that pCD affects multiple stages of tumor progression including proliferation, angiogenesis and metastasis. Previously, we showed that the mitogenic effect of pCD on cancer cells is mediated by interaction of its activation peptide (AP) with yet unidentified cell surface receptor. In this investigation, gene expression profiles were compared between AP-treated and control human breast cancer ZR-75-1 cells to elucidate the mechanism of AP mitogenicity. Several differentially expressed genes involved in signal transduction, regulation of cell cycle, apoptosis, tumor invasion and metastasis were identified using microarray technology. These findings, including overexpression of NF-kappaB2, were confirmed in breast cancer cell lines by reverse-transcriptase PCR (RT-PCR). Understanding the mechanism of pCDs effect on breast cancer cells could extend possibilities of breast cancer treatment in the future.
References provided by Crossref.org
Cathepsin D--many functions of one aspartic protease
