Overexpression of procathepsin D (pCD) is reported to occur in numerous types of cancer and is associated with increased growth and metastasis. It has been established that pCD affects multiple stages of tumor progression including proliferation, angiogenesis and metastasis. Previously, we showed that the mitogenic effect of pCD on cancer cells is mediated by interaction of its activation peptide (AP) with yet unidentified cell surface receptor. In this investigation, gene expression profiles were compared between AP-treated and control human breast cancer ZR-75-1 cells to elucidate the mechanism of AP mitogenicity. Several differentially expressed genes involved in signal transduction, regulation of cell cycle, apoptosis, tumor invasion and metastasis were identified using microarray technology. These findings, including overexpression of NF-kappaB2, were confirmed in breast cancer cell lines by reverse-transcriptase PCR (RT-PCR). Understanding the mechanism of pCDs effect on breast cancer cells could extend possibilities of breast cancer treatment in the future.

• MeSH
• financování organizované MeSH
• kathepsin D farmakologie   MeSH
• lidé MeSH
• nádorové buňky kultivované MeSH
• nádorové proteiny genetika   MeSH
• nádory prsu genetika   MeSH
• NF-kappa B - podjednotka p52 genetika   metabolismus   MeSH
• peptidové fragmenty farmakologie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• prekurzory enzymů farmakologie   MeSH
• regulace genové exprese u nádorů účinky léků   MeSH
• sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
• signální transdukce MeSH
• stanovení celkové genové exprese MeSH
• Check Tag
• lidé MeSH