Adenosine potentiates stimulatory effects on granulocyte-macrophage hematopoietic progenitor cells in vitro of IL-3 and SCF, but not those of G-CSF, GM-CSF and IL-11
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16343044
DOI
10.33549/physiolres.930854
PII: 854
Knihovny.cz E-resources
- MeSH
- Adenosine pharmacology MeSH
- Bone Marrow Cells cytology drug effects MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor pharmacology MeSH
- Granulocyte Colony-Stimulating Factor pharmacology MeSH
- Colony-Stimulating Factors pharmacology MeSH
- Granulocytes cytology drug effects MeSH
- Hematopoietic Stem Cells cytology drug effects MeSH
- Interleukin-11 pharmacology MeSH
- Interleukin-3 pharmacology MeSH
- Cells, Cultured MeSH
- Macrophages cytology drug effects MeSH
- Mice, Inbred C57BL MeSH
- Mice, Inbred CBA MeSH
- Mice MeSH
- Cell Proliferation drug effects MeSH
- Stem Cell Factor pharmacology MeSH
- Drug Synergism MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor MeSH
- Granulocyte Colony-Stimulating Factor MeSH
- Colony-Stimulating Factors MeSH
- Interleukin-11 MeSH
- Interleukin-3 MeSH
- Stem Cell Factor MeSH
The aim of the studies was to ascertain if adenosine is able to co-operate with selected hematopoietic growth factors and cytokines, namely with granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), interleukin-3 (IL-3), and interleukin-11 (IL-11), in inducing the growth of colonies from hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC) from normal bone marrow cells in vitro. Adenosine was found not to produce any colonies when present in the cultures as the only potential stimulator. All the tested cytokines and growth factors were observed to induce the growth of distinct numbers of GM-CFC colonies, with the exception of IL-11. When suboptimal concentrations of the evaluated cytokines and growth factors were tested in the cultures in which various concentrations of adenosine were concomitantly present, mutually potentiating effects were found in the case of IL-3 and SCF. These results confirm the role of adenosine in regulation of granulopoiesis and predict IL-3 and SCF as candidates for further in vivo studies of their combined administration with adenosine.
References provided by Crossref.org
The role of adenosine receptor agonists in regulation of hematopoiesis
