Major histocompatibility complex (MHC) genes play important role in host-parasite interactions and parasites are crucial factors influencing the population dynamics of hosts. We described the structure and diversity of exon 2 of the MHC class II DQA gene in three species of voles (Arvicolinae) exhibiting regular multi-annual fluctuations of population density and analysed the processes leading to the observed MHC polymorphism. By using cloning-sequencing methodology and capillary electrophoresis-single strand conformation polymorphism, we described seven sequences in the water, eight in the common, and seven in the bank voles coming from an area of 70 km(2) around the Nozeroy canton in the Jura Mountains (Franche Comté, France). All exon 2 sequences translate to give unique amino acid sequences and positive selection was found to act very intensively on antigen binding sites. We documented the presence of recombination at vole DQA region but its importance in generating allelic polymorphism seems to be relatively limited. For the first time within rodents, we documented the duplication of the DQA gene in all three species with both copies being transcriptionally active. Phylogenetic analysis of allelic sequences revealed extensive trans-species polymorphism within the subfamily although no alleles were shared between species in our data set. We discuss possible role of parasites in forming the recent polymorphism pattern of the DQA locus in voles.

Centre de Biologie et Gestion des Populations INRA Campus International de Baillarguet CS 30016 34988 Montferrier sur Lez Cedex France

Zobrazit více v PubMed

Mol Phylogenet Evol. 2004 Dec;33(3):647-63 PubMed

Nature. 1993 Jul 1;364(6432):33-9 PubMed

Genome Res. 2004 Apr;14(4):631-9 PubMed

Immunogenetics. 2003 Aug;55(5):344-50 PubMed

Immunogenetics. 1990;31(4):217-9 PubMed

Philos Trans R Soc Lond B Biol Sci. 1994 Nov 29;346(1317):359-66; discussion 366-7 PubMed

Immunity. 2000 Jun;12(6):699-710 PubMed

Immunol Today. 1994 Feb;15(2):58-62 PubMed

Syst Biol. 2004 Aug;53(4):533-53 PubMed

Mol Ecol. 1999 Dec;8(12):2071-9 PubMed

Immunogenetics. 2005 Apr;57(1-2):108-15 PubMed

Proc Natl Acad Sci U S A. 1990 Mar;87(6):2264-8 PubMed

Heredity (Edinb). 2000 Dec;85(Pt 6):617-24 PubMed

Am J Hum Genet. 1992 Jan;50(1):1-5 PubMed

Hum Immunol. 1987 Jul;19(3):155-62 PubMed

Nature. 1999 Oct 28;401(6756):921-3 PubMed

Immunol Rev. 1999 Feb;167:319-25 PubMed

Mol Phylogenet Evol. 2000 Jul;16(1):37-47 PubMed

Immunogenetics. 1998 Jul;48(2):161-2 PubMed

Genetics. 2002 Mar;160(3):1231-41 PubMed

Immunity. 1998 Mar;8(3):305-17 PubMed

Crit Rev Immunol. 1997;17(2):179-224 PubMed

Mol Biol Evol. 2000 May;17(5):768-72 PubMed

Immunogenetics. 2002 Oct;54(7):490-500 PubMed

Eur J Immunogenet. 2002 Jun;29(3):213-7 PubMed

Genetics. 2001 Dec;159(4):1805-17 PubMed

Int J Parasitol. 1998 Apr;28(4):537-42 PubMed

Nature. 1988 Sep 15;335(6187):265-7 PubMed

Mol Biol Evol. 2000 Oct;17(10):1446-55 PubMed

Biotechniques. 2002 Dec;33(6):1342-8 PubMed

Immunogenetics. 1998 Oct;48(5):344-9 PubMed

Philos Trans R Soc Lond B Biol Sci. 1994 Nov 29;346(1317):351-7; discussion 357-8 PubMed

Evolution. 2000 Dec;54(6):2145-51 PubMed

Mol Biol Evol. 2005 Apr;22(4):1107-18 PubMed

Immunogenetics. 1997;46(3):237-44 PubMed

Mol Ecol. 1999 Aug;8(8):1259-72 PubMed

Tissue Antigens. 1999 Dec;54(6):615-24 PubMed

Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12200-4 PubMed

Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3714-9 PubMed

Annu Rev Genet. 1998;32:415-35 PubMed

Hum Genet. 2001 Mar;108(3):249-54 PubMed

Mol Biol Evol. 1990 Nov;7(6):491-514 PubMed

Clin Exp Immunol. 2000 Sep;121(3):491-8 PubMed

Genetics. 1990 Apr;124(4):967-78 PubMed

Immunogenetics. 2004 Sep;56(6):420-6 PubMed

Front Biosci. 1998 May 26;3:d509-16 PubMed

Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7799-806 PubMed

Mol Ecol. 2005 Aug;14(9):2861-71 PubMed

Syst Parasitol. 2003 Jan;54(1):53-69 PubMed

Immunogenetics. 1988;27(2):110-20 PubMed

Mol Ecol. 2005 Jun;14(7):2233-43 PubMed

Mol Phylogenet Evol. 2000 Nov;17(2):280-93 PubMed

Genetics. 2003 May;164(1):289-97 PubMed

Nucleic Acids Res. 2003 Jan 1;31(1):193-5 PubMed

Immunogenetics. 2004 Sep;56(6):427-37 PubMed

Immunogenetics. 2004 Apr;56(1):12-27 PubMed

Genetica. 1998-1999;104(3):301-9 PubMed

Genetics. 1999 May;152(1):413-25 PubMed

Comput Appl Biosci. 1997 Oct;13(5):555-6 PubMed

Immunogenetics. 2002 Jul;54(4):230-9 PubMed

Mol Ecol. 2005 Jan;14(1):85-91 PubMed

Evolution. 2005 Feb;59(2):439-50 PubMed

Int J Parasitol. 2003 Sep 30;33(11):1219-31 PubMed

Immunogenetics. 2002 Aug;54(5):348-52 PubMed

Brief Bioinform. 2004 Jun;5(2):150-63 PubMed

Fish Shellfish Immunol. 2005 Mar;18(3):199-222 PubMed

Genetics. 1997 Jun;146(2):655-68 PubMed

Nucleic Acids Res. 2005 Jan 1;33(Database issue):D523-6 PubMed

Genet Res. 2003 Oct;82(2):89-99 PubMed

Immunogenetics. 1999 Nov;50(3-4):168-200 PubMed

Mol Biol Evol. 1986 Sep;3(5):418-26 PubMed

Br Vet J. 1995 Mar-Apr;151(2):119-40 PubMed

Mol Biol Evol. 2002 May;19(5):708-17 PubMed

Immunogenetics. 2001 Sep;53(7):520-42 PubMed

Mol Ecol. 2001 Aug;10(8):1939-45 PubMed

Proc Biol Sci. 1994 May 23;256(1346):119-24 PubMed

Immunity. 1998 Mar;8(3):319-29 PubMed

Immunogenetics. 1999 Sep;49(10):886-93 PubMed

Anim Genet. 1998 Oct;29(5):356-62 PubMed

Proc Biol Sci. 2004 Apr 22;271(1541):859-67 PubMed

Anim Genet. 1993 Aug;24(4):249-55 PubMed

Genetics. 2000 Oct;156(2):879-91 PubMed

Genetic diversity, evolution and selection in the major histocompatibility complex DRB and DQB loci in the family Equidae

De novo transcriptome assembly facilitates characterisation of fast-evolving gene families, MHC class I in the bank vole (Myodes glareolus)

Contrasted evolutionary histories of two Toll-like receptors (Tlr4 and Tlr7) in wild rodents (MURINAE)

Genetic structure and contrasting selection pattern at two major histocompatibility complex genes in wild house mouse populations

Structure, diversity and evolutionary patterns of expressed MHC class IIB genes in chub (Squalius cephalus), a cyprinid fish species from Europe

Negative relationships between cellular immune response, Mhc class II heterozygosity and secondary sexual trait in the montane water vole

Polymorphism and selection in the major histocompatibility complex DRA and DQA genes in the family Equidae