Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16524412
DOI
10.1111/j.1469-0691.2006.01365.x
PII: S1198-743X(14)61603-7
Knihovny.cz E-resources
- MeSH
- Enterococcus faecalis classification isolation & purification MeSH
- Enterococcus faecium classification genetics isolation & purification MeSH
- Genotype MeSH
- Gram-Positive Bacterial Infections microbiology MeSH
- Hematologic Neoplasms MeSH
- Cross Infection microbiology MeSH
- Humans MeSH
- Neoplasms MeSH
- Cancer Care Facilities * MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Vancomycin Resistance genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. The results indicated potential survival of strains in the hospital environment and possible subsequent transmission to hospitalised patients.
References provided by Crossref.org
Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
