This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. The results indicated potential survival of strains in the hospital environment and possible subsequent transmission to hospitalised patients.

• MeSH
• Enterococcus faecalis izolace a purifikace   klasifikace   MeSH
• Enterococcus faecium genetika   izolace a purifikace   klasifikace   MeSH
• financování organizované MeSH
• grampozitivní bakteriální infekce mikrobiologie   MeSH
• hematologické nádory MeSH
• infekce spojené se zdravotní péčí mikrobiologie   MeSH
• lidé MeSH
• nádory MeSH
• onkologická péče - zařízení MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• rezistence na vankomycin genetika   MeSH
• Check Tag
• lidé MeSH
• Geografické názvy
• Česká republika MeSH

Occurrence of vancomycin-resistant enterococci and its relationship to the administration of glycopeptide antibiotics (vancomycin, teicoplanin) was described. A total number of 628 strains of the genus Enterococcus was isolated in the Hemato-oncologic Department of the University Hospital in Olomouc in 1997. Seven strains (1.1%) were found to be resistant to vancomycin. Five of them were identified as Enterococcus faecalis of phenotype VanB, and two strains as E. faecium of phenotype VanA. The administration of vancomycin and teicoplanin in the Hemato-oncologic Department amounted to 85.7% and 89.2%, respectively, of the total consumption of these drugs at the University Hospital.

• MeSH
• antibakteriální látky farmakologie   terapeutické užití   MeSH
• Enterococcus faecalis klasifikace   účinky léků   izolace a purifikace   MeSH
• Enterococcus faecium klasifikace   účinky léků   izolace a purifikace   MeSH
• Enterococcus klasifikace   účinky léků   izolace a purifikace   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• rezistence na vankomycin * MeSH
• teikoplanin farmakologie   terapeutické užití   MeSH
• vankomycin farmakologie   terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH