Analysis of insect cuticular hydrocarbons using matrix-assisted laser desorption/ionization mass spectrometry
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chloroform chemistry MeSH
- Insecta physiology MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Hydrocarbons analysis metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chloroform MeSH
- Hydrocarbons MeSH
Insect cuticular hydrocarbons (CHCs) were probed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry with a lithium 2,5-dihydroxybenzoate matrix. CHC profiles were obtained for 12 species of diverse insect taxa (termites, ants, a cockroach, and a flesh fly). MALDI spectra revealed the presence of high molecular weight CHCs on the insect cuticle. Hydrocarbons with more than 70 carbon atoms, both saturated and unsaturated, were detected. When compared with gas chromatography/mass spectrometry (GC/MS), MALDI-TOF covered a wider range of CHCs and enabled CHCs of considerably higher molecular weight to be detected. Good congruity between GC/MS and MALDI-TOF was observed in the overlapping region of molecular weights. Moreover, a number of previously undiscovered hydrocarbons were detected in the high mass range beyond the analytical capabilities of current GC/MS instruments. MALDI was shown to hold potential to become an alternative analytical method for insect CHC analyses. The ability of MALDI to discriminate among species varying in the degree of their relatedness was found to be similar to GC/MS. However, neither MALDI-MS nor GC/MS data were able to describe the phylogenetic relationships.
See more in PubMed
Anal Chem. 2000 Jan 1;72(1):101-9 PubMed
Rapid Commun Mass Spectrom. 2002;16(3):208-11 PubMed
J Am Soc Mass Spectrom. 2004 Feb;15(2):168-79 PubMed
J Chem Ecol. 1995 Nov;21(11):1837-45 PubMed
Anal Chem. 2002 Aug 1;74(15):3911-4 PubMed
J Chem Ecol. 1995 Nov;21(11):1677-95 PubMed
Anal Chem. 2001 Mar 1;73(5):864-9 PubMed
J Chem Ecol. 1996 Apr;22(4):637-51 PubMed
J Chem Ecol. 1990 May;16(5):1635-47 PubMed
J Insect Physiol. 1997 Nov;44(1):59-66 PubMed
Rapid Commun Mass Spectrom. 2003;17 (19):2203-7 PubMed
Annu Rev Entomol. 2001;46:573-99 PubMed
Annu Rev Entomol. 2005;50:371-93 PubMed
J Insect Physiol. 1975 Nov;21(11):1827-33 PubMed
Evolution. 1987 Mar;41(2):280-293 PubMed
Mol Ecol. 2004 Oct;13(10):3099-113 PubMed
J Chem Ecol. 1990 Oct;16(10):2835-58 PubMed
Tetrahedron. 1970 Jan;26(2):307-19 PubMed
Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives
MALDI imaging of neutral cuticular lipids in insects and plants
