The spatial distribution of neutral lipids and hydrocarbons has been imaged using MALDI-TOF mass spectrometry on intact plant and insect surfaces, namely wings and legs of the gray flesh fly (Neobellieria bullata), wings of common fruit fly (Drosophila melanogaster), leaves of thale cress (Arabidopsis thaliana), and leaves of date palm tree (Phoenix sp.). The distribution of wax esters (WEs) and saturated and unsaturated hydrocarbons (HCs) was visualized. The samples were attached on a target and multiply sprayed with lithium or sodium 2,5-dihydroxybenzoate. The deposits were homogenous, consisting of small islands (50-150 microm) of matrix crystals separated by small areas (10 microm) of uncovered cuticle. Samples of N. bullata wings were found to contain HCs and WEs distributed close to their basal parts. The distribution of sodium and potassium ions was visualized on samples prepared by sublimation of 2,5-dihydroxybenzoic acid. Pheromonal dienes were detected on D. melanogaster female wings. A homogenous distribution of saturated WEs was observed on A. thaliana and Phoenix sp. leaf samples. The optimum number of laser shots per pixel was found to be higher than for polar compounds imaging.

Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic Prague Czech Republic

Zobrazit více v PubMed

J Lipid Res. 2008 Feb;49(2):438-54 PubMed

Anal Chem. 2004 Mar 15;76(6):1657-63 PubMed

Prog Lipid Res. 2004 Sep;43(5):449-88 PubMed

J Insect Physiol. 1975 Nov;21(11):1827-33 PubMed

Anal Chem. 2005 Oct 15;77(20):6682-6 PubMed

Annu Rev Entomol. 2005;50:371-93 PubMed

J Am Soc Mass Spectrom. 2008 Jun;19(6):882-6 PubMed

Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6196-201 PubMed

Bioessays. 1997 Apr;19(4):353-8 PubMed

Plant J. 2007 Apr;50(2):189-96 PubMed

Prog Lipid Res. 2003 Jan;42(1):51-80 PubMed

Electrophoresis. 2002 Sep;23(18):3125-35 PubMed

Mass Spectrom Rev. 2007 Jul-Aug;26(4):606-43 PubMed

J Mass Spectrom. 2009 Jan;44(1):101-10 PubMed

Anal Chem. 2006 May 1;78(9):3062-71 PubMed

Anal Chem. 2006 Sep 15;78(18):6448-56 PubMed

Proc Natl Acad Sci U S A. 2008 May 20;105(20):7135-40 PubMed

Planta. 2001 Jul;213(3):427-34 PubMed

Lipids. 1971 Jul;6(7):502-7 PubMed

Rapid Commun Mass Spectrom. 2008 May;22(10):1503-9 PubMed

Anal Chem. 1997 Dec 1;69(23):4751-60 PubMed

J Chem Ecol. 2006 Feb;32(2):409-34 PubMed

Annu Rev Plant Biol. 2008;59:683-707 PubMed

J Am Soc Mass Spectrom. 2007 Sep;18(9):1646-52 PubMed

AAPS J. 2006 Jun 02;8(2):E391-5 PubMed

Anal Chem. 2008 Apr 15;80(8):2780-8 PubMed

Rapid Commun Mass Spectrom. 2006;20(6):965-72 PubMed

J Mass Spectrom. 2003 Jul;38(7):699-708 PubMed

Evolution. 1987 Mar;41(2):294-302 PubMed

Rapid Commun Mass Spectrom. 2003;17(19):2203-7 PubMed

Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:405-430 PubMed

J Am Chem Soc. 2005 Sep 7;127(35):12152-3 PubMed

Silver Ion High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry: A Tool for Analyzing Cuticular Hydrocarbons

Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives

Cuticular hydrocarbons of the South American fruit fly Anastrepha fraterculus: variability with sex and age

Molecular mass spectrometry imaging in biomedical and life science research