MALDI imaging of neutral cuticular lipids in insects and plants
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19910210
DOI
10.1016/j.jasms.2009.10.003
PII: S1044-0305(09)00772-7
Knihovny.cz E-resources
- MeSH
- Arabidopsis anatomy & histology chemistry MeSH
- Arecaceae anatomy & histology MeSH
- Diptera anatomy & histology chemistry MeSH
- Potassium chemistry MeSH
- Drosophila melanogaster anatomy & histology MeSH
- Photography MeSH
- Gentisates chemistry MeSH
- Insect Proteins chemistry MeSH
- Wings, Animal anatomy & histology chemistry MeSH
- Lipids analysis chemistry MeSH
- Plant Leaves chemistry MeSH
- Lithium chemistry MeSH
- Image Processing, Computer-Assisted methods MeSH
- Plant Proteins chemistry MeSH
- Sodium chemistry MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Hydrocarbons chemistry MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2,5-dihydroxybenzoic acid MeSH Browser
- Potassium MeSH
- Gentisates MeSH
- Insect Proteins MeSH
- Lipids MeSH
- Lithium MeSH
- Plant Proteins MeSH
- Sodium MeSH
- Hydrocarbons MeSH
The spatial distribution of neutral lipids and hydrocarbons has been imaged using MALDI-TOF mass spectrometry on intact plant and insect surfaces, namely wings and legs of the gray flesh fly (Neobellieria bullata), wings of common fruit fly (Drosophila melanogaster), leaves of thale cress (Arabidopsis thaliana), and leaves of date palm tree (Phoenix sp.). The distribution of wax esters (WEs) and saturated and unsaturated hydrocarbons (HCs) was visualized. The samples were attached on a target and multiply sprayed with lithium or sodium 2,5-dihydroxybenzoate. The deposits were homogenous, consisting of small islands (50-150 microm) of matrix crystals separated by small areas (10 microm) of uncovered cuticle. Samples of N. bullata wings were found to contain HCs and WEs distributed close to their basal parts. The distribution of sodium and potassium ions was visualized on samples prepared by sublimation of 2,5-dihydroxybenzoic acid. Pheromonal dienes were detected on D. melanogaster female wings. A homogenous distribution of saturated WEs was observed on A. thaliana and Phoenix sp. leaf samples. The optimum number of laser shots per pixel was found to be higher than for polar compounds imaging.
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