Cytokine and inflammatory response against virulent LT2 strain and its attenuated aroA deletion mutant of Salmonella enterica serovar Typhimurium were compared in gnotobiotic pigs. Contrary to the parental strain, the auxotrofic mutant did not induce IL-1beta, IL-18, TNF-alpha, and IFN-gamma in the ileum and plasma 24h after the infection, did not cause pathological changes in ileal epithelium and mesenteric lymph nodes or immunoreactivity of gp91 phox and peroxynitrite and was not immunostained for GroEL stress protein. The absence of induction of proinflammatory cytokines may be a reason why aroA mutant was unable to elicit any inflammatory response and protect pigs against challenge with virulent LT2 strain administered 24h later.

Department of Immunology and Gnotobiology Institute of Microbiology Academy of Sciences of the Czech Republic 549 22 Nový Hrádek Czech Republic

References provided by Crossref.org

Toll-Like Receptor 4 Signaling in the Ileum and Colon of Gnotobiotic Piglets Infected with Salmonella Typhimurium or Its Isogenic ∆rfa Mutants

Impact of the Lipopolysaccharide Chemotype of Salmonella Enterica Serovar Typhimurium on Virulence in Gnotobiotic Piglets

Innate immune response in the gut against Salmonella - review

aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity