Multiplex PCR for detection of three exfoliative toxin serotype genes in Staphylococcus aureus
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16681147
DOI
10.1007/bf02931437
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial analysis MeSH
- Adult MeSH
- Exfoliatins genetics MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Polymerase Chain Reaction methods MeSH
- Serotyping MeSH
- Staphylococcal Skin Infections microbiology MeSH
- Staphylococcus aureus classification genetics isolation & purification MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Exfoliatins MeSH
Rapid and specific detection of exfoliative toxin (ET)-producing Staphylococcus aureus strains by multiplex polymerase chain reaction (PCR) was used for identification of exfoliative toxin genes in a diverse set of 115 clinical S. aureus strains isolated in 14 Czech cities between 1998 and 2004. Fifty-nine wild-type ET-positive isolates of which 40 strains were the causative agents of toxic epidermolysis in neonates were classified into 4 PCR types. The genes coding for ETA, ETB or ETD were not detected in any of non-ET-producing isolates. The PCR method using the multiplex and specific primer set was shown to be reliable in rapid identification of the exfoliative toxin producing S. aureus and can be used as a convenient tool for hospital epidermolytic infection control.
See more in PubMed
Appl Environ Microbiol. 1986 May;51(5):885-90 PubMed
Int J Med Microbiol. 2003 Feb;292(7-8):541-5 PubMed
Mol Microbiol. 2000 Nov;38(4):694-705 PubMed
J Clin Microbiol. 2000 Mar;38(3):1032-5 PubMed
J Clin Microbiol. 1998 Sep;36(9):2548-53 PubMed
Infect Immun. 1980 Nov;30(2):601-6 PubMed
Mol Cell Probes. 2001 Oct;15(5):249-57 PubMed
Infect Immun. 2002 Oct;70(10):5835-45 PubMed
J Clin Microbiol. 1975 May;1(5):397-400 PubMed
J Invest Dermatol. 2004 May;122(5):1070-7 PubMed
Infect Immun. 2001 Dec;69(12):7760-71 PubMed
FEMS Immunol Med Microbiol. 1998 Apr;20(4):301-10 PubMed
J Clin Microbiol. 1991 Mar;29(3):426-30 PubMed
Biotechniques. 1997 Sep;23(3):504-11 PubMed
Clin Microbiol Rev. 1999 Apr;12(2):224-42 PubMed
Vet Microbiol. 2003 Oct 8;96(1):81-90 PubMed
Folia Microbiol (Praha). 2004;49(4):353-86 PubMed
Microbiol Immunol. 1995;39(6):379-86 PubMed
Infect Immun. 1994 Sep;62(9):3780-5 PubMed
J Bacteriol. 1987 Sep;169(9):3904-9 PubMed
Oxacillin (Methicillin) Resistant Staphylococci in Domestic Animals in the Czech Republic
Characteristics of Staphylococcus aureus isolated from rabbits
Development of specific and rapid detection of bacterial pathogens in dairy products by PCR
