Multiplex PCR for detection of three exfoliative toxin serotype genes in Staphylococcus aureus
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
16681147
DOI
10.1007/bf02931437
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií analýza MeSH
- dospělí MeSH
- exfoliatiny genetika MeSH
- lidé MeSH
- novorozenec MeSH
- polymerázová řetězová reakce metody MeSH
- sérotypizace MeSH
- stafylokokové infekce kůže mikrobiologie MeSH
- Staphylococcus aureus klasifikace genetika izolace a purifikace MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA bakterií MeSH
- exfoliatiny MeSH
Rapid and specific detection of exfoliative toxin (ET)-producing Staphylococcus aureus strains by multiplex polymerase chain reaction (PCR) was used for identification of exfoliative toxin genes in a diverse set of 115 clinical S. aureus strains isolated in 14 Czech cities between 1998 and 2004. Fifty-nine wild-type ET-positive isolates of which 40 strains were the causative agents of toxic epidermolysis in neonates were classified into 4 PCR types. The genes coding for ETA, ETB or ETD were not detected in any of non-ET-producing isolates. The PCR method using the multiplex and specific primer set was shown to be reliable in rapid identification of the exfoliative toxin producing S. aureus and can be used as a convenient tool for hospital epidermolytic infection control.
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