HPLC determination of a novel aroylhydrazone iron chelator (o-108) in rabbit plasma and its application to a pilot pharmacokinetic study
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16725389
DOI
10.1016/j.jchromb.2006.04.020
PII: S1570-0232(06)00355-2
Knihovny.cz E-resources
- MeSH
- Iron Chelating Agents analysis chemistry pharmacokinetics MeSH
- Hydrazones blood chemistry pharmacokinetics MeSH
- Rabbits MeSH
- Molecular Structure MeSH
- Pilot Projects MeSH
- Area Under Curve MeSH
- Pyridoxal analogs & derivatives blood pharmacokinetics MeSH
- Reproducibility of Results MeSH
- Drug Stability MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Iron Chelating Agents MeSH
- Hydrazones MeSH
- pyridoxal 2-chlorobenzoyl hydrazone MeSH Browser
- Pyridoxal MeSH
A high performance liquid chromatographic method for the determination of a biocompatible iron chelator, pyridoxal 2-chlorobenzoyl hydrazone (o-108), in rabbit plasma was developed and validated. The separation was achieved on a C18 column with the mobile phase composed of a mixture of 0.01 M phosphate buffer (pH 6) with the addition of EDTA (2 mM), methanol and acetonitrile (42:24:14; v/v/v). The method was validated with respect to selectivity, linearity (0.8-150 microg/mL), intra- and inter-day variability and stability. This method was successfully applied to the analysis of the samples obtained from a pilot pharmacokinetic experiment, in which the chelator was administered intravenously to rabbits.
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