Effect of free radical spin trap N-tert-butyl-alpha-phenylnitrone (PBN) on seizures induced in immature rats by homocysteic acid
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16756975
DOI
10.1016/j.expneurol.2006.03.031
PII: S0014-4886(06)00243-3
Knihovny.cz E-zdroje
- MeSH
- adenosintrifosfát metabolismus MeSH
- disacharidy aplikace a dávkování farmakologie MeSH
- elektroencefalografie MeSH
- energetický metabolismus účinky léků MeSH
- fosfokreatin metabolismus MeSH
- glukosa metabolismus MeSH
- glykogen metabolismus MeSH
- hipokampus účinky léků metabolismus patologie MeSH
- homocystein analogy a deriváty toxicita MeSH
- injekce intraperitoneální MeSH
- krysa rodu Rattus MeSH
- mozek účinky léků metabolismus patologie MeSH
- mozková kůra účinky léků metabolismus patologie MeSH
- neurony účinky léků metabolismus patologie MeSH
- potkani Wistar MeSH
- spin trapping MeSH
- volné radikály antagonisté a inhibitory MeSH
- záchvaty chemicky indukované patofyziologie prevence a kontrola MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenosintrifosfát MeSH
- disacharidy MeSH
- fosfokreatin MeSH
- glukosa MeSH
- glykogen MeSH
- homocysteic acid MeSH Prohlížeč
- homocystein MeSH
- PBNLP compound MeSH Prohlížeč
- volné radikály MeSH
The present study has examined the effect of free radical spin trap N-tert-butyl-alpha-phenylnitrone (PBN) in the model of seizures induced in immature 12-day-old rats by bilateral intracerebroventricular infusion of dl-homocysteic acid (dl-HCA, 600 nmol/side). PBN was given i.p. in two doses (100 mg/kg each), 30 min prior and 30 min after dl-HCA infusion. PBN did not significantly influence the severity of seizures, evident both from the behavioral symptoms and EEG recordings. PBN normalized decreased ATP levels in the hippocampus, occurring during the acute phase of seizures ( approximately 45-50 min after infusion) and persisting until the end of the 24-h recovery period. PBN also led to normalization of decreased glucose levels and to a significant reduction of lactate accumulation in the cerebral cortex and hippocampus. The neuroprotective effect of PBN was evaluated after 24 h and 6 days of survival following dl-HCA-induced seizures (Nissl and Fluoro-Jade B staining). The administration of PBN resulted in a partial amelioration of severe damage observed in many brain regions following infusion of dl-HCA alone. The data suggest that increased free radical production is apparently occurring during seizures induced in immature rats by homocysteic acid. Free radical scavenger PBN had a clear-cut protective effect, evident as the improved recovery of brain energy status and as a partial, but significant, attenuation of neuronal degeneration associated with this model of seizures.
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