Certain aspects of uncoupling due to mitochondrial uncoupling proteins in vitro and in vivo
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
16781660
DOI
10.1016/j.bbabio.2006.05.005
PII: S0005-2728(06)00129-0
Knihovny.cz E-resources
- MeSH
- Cell Respiration MeSH
- Guanosine Triphosphate metabolism MeSH
- Adipose Tissue, Brown metabolism MeSH
- Ion Channels MeSH
- Carnitine metabolism MeSH
- Plant Roots metabolism MeSH
- Zea mays metabolism MeSH
- Lauric Acids metabolism pharmacology MeSH
- Fatty Acids metabolism MeSH
- Membrane Proteins physiology MeSH
- Mitochondrial Proteins MeSH
- Mitochondria metabolism MeSH
- Oxidative Stress MeSH
- Hydrogen Peroxide metabolism MeSH
- Reactive Oxygen Species metabolism MeSH
- Plant Proteins physiology MeSH
- Uncoupling Agents metabolism pharmacology MeSH
- Mammals MeSH
- In Vitro Techniques MeSH
- Carrier Proteins physiology MeSH
- Uncoupling Protein 1 MeSH
- Binding Sites MeSH
- Plant Shoots metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Guanosine Triphosphate MeSH
- Ion Channels MeSH
- Carnitine MeSH
- Lauric Acids MeSH
- lauric acid MeSH Browser
- Fatty Acids MeSH
- Membrane Proteins MeSH
- Mitochondrial Proteins MeSH
- Hydrogen Peroxide MeSH
- Reactive Oxygen Species MeSH
- Plant Proteins MeSH
- Uncoupling Agents MeSH
- Carrier Proteins MeSH
- Uncoupling Protein 1 MeSH
Thermogenic uncoupling has been proven only for UCP1 in brown adipose tissue. All other isoforms of UCPs are potentially acting in suppression of mitochondrial reactive oxygen species (ROS) production. In this contribution we show that BAT mitochondria can be uncoupled by lauric acid in the range of approximately 100 nM when endogenous fatty acids are combusted by carnitine cycle and beta-oxidation is properly separated from the uncoupling effect. Respiration increased up to 3 times when related to the lowest fatty acid content (BSA present plus carnitine cycle). We also illustrated that any effect leading to more coupled states leads to enhanced H2O2 generation and any effect resulting in uncoupling gives reduced H2O2 generation in BAT mitochondria. Finally, we report doubling of plant UCP transcript in cells as well as amount of protein detected by 3H-GTP-binding sites in mitochondria of shoots and roots of maize seedlings subjected to the salt stress.
References provided by Crossref.org
Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling