Effect of bacterial monoassociation on brush-border enzyme activities in ex-germ-free piglets: comparison of commensal and pathogenic Escherichia coli strains
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
16949322
DOI
10.1016/j.micinf.2006.07.008
PII: S1286-4579(06)00269-3
Knihovny.cz E-zdroje
- MeSH
- alkalická fosfatasa analýza MeSH
- antigeny CD13 analýza MeSH
- bakteriální adheze * MeSH
- Escherichia coli patogenita fyziologie MeSH
- gama-glutamyltransferasa analýza MeSH
- glukoamylasa analýza MeSH
- gnotobiologické modely MeSH
- histocytochemie MeSH
- laktasa analýza MeSH
- mikroklky enzymologie MeSH
- prasata MeSH
- sacharasa analýza MeSH
- tenké střevo enzymologie mikrobiologie MeSH
- TNF-alfa krev MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- alkalická fosfatasa MeSH
- antigeny CD13 MeSH
- gama-glutamyltransferasa MeSH
- glukoamylasa MeSH
- laktasa MeSH
- sacharasa MeSH
- TNF-alfa MeSH
This study was designed to investigate the effect of monoassociation of germ-free piglets with Escherichia coli strains on the development of intestinal brush-border enzyme activities. Piglets were delivered by hysterectomy, reared for seven days under germ-free conditions and fed milk formula diet. One group was maintained germ-free, the other four groups were monoassociated on day eight with one of four E. coli strains: non-pathogenic O86 or O83 and G58-1, or pathogenic 933D. The development of brush-border digestive enzyme functions in the small intestine was evaluated after 15 days. Germ-free controls exhibited slower developmental declines of lactase, gamma-glutamyltranspeptidase and alkaline phosphatase, and delayed increases of sucrase and glucoamylase compared to conventionally grown animals. Association of germ-free piglets with the non-pathogenic E. coli strains O86 and O83 resulted in increased enterocyte differentiation along the length of the small intestine, accompanied by declining activities of lactase, gamma-glutamyltranspeptidase and alkaline phosphatase, and elevated activities of maturational markers such as sucrase and glucoamylase. Maturational changes also occurred along the villus-crypt axis, as revealed by histochemical localization of aminopeptidase N on the villi tips in piglets colonized with E. coli O83. Interestingly, colonization with the pathogenic E. coli strain 933D stimulated changes in the main differentiation enzyme markers lactase, sucrase and glucoamylase to an extent comparable with those produced by the non-pathogenic and probiotic E. coli strains. In conclusion, germ-free piglets represent a valuable tool to study the consequences of colonization of the immature sterile gut with defined strains of bacteria.
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