Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-acetyltransferase in Zygosaccharomyces rouxii confers resistance to L-azetidine-2-carboxylate
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17004651
DOI
10.1007/bf02932123
Knihovny.cz E-resources
- MeSH
- Acetyltransferases genetics metabolism MeSH
- Drug Resistance, Microbial genetics MeSH
- Transformation, Bacterial MeSH
- Genetic Engineering MeSH
- Genetic Markers MeSH
- Culture Media chemistry MeSH
- Azetidinecarboxylic Acid antagonists & inhibitors pharmacology MeSH
- Saccharomyces cerevisiae Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae genetics physiology MeSH
- Selection, Genetic MeSH
- Gene Transfer Techniques MeSH
- Zygosaccharomyces genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acetyltransferases MeSH
- Genetic Markers MeSH
- Culture Media MeSH
- Azetidinecarboxylic Acid MeSH
- Mpr1 protein, S cerevisiae MeSH Browser
- Saccharomyces cerevisiae Proteins MeSH
The osmotolerant yeast Zygosaccharomyces rouxii is sensitive to the toxic L-proline analogue, L-azetidine-2-carboxylate (AZC). The possibility of use of the Saccharomyces cerevisiae MPR1 gene (ScMPR1) encoding the AZC-detoxifying enzyme as a dominant selection marker in Z. rouxii was examined. The heterologous expression of ScMPR1 in two Z. rouxii strains resulted in AZC-resistant colonies, but that of ScMPR1 as a dominant marker gene in vectors was affected by a high frequency of spontaneously resistant colonies. The same was found for an AZC-sensitive S. cerevisiae strain in which the ScMPR1 was expressed. In both yeasts, ScMPR1 can be used only as an auxiliary marker gene.
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