Impairment of muscarinic transmission in transgenic APPswe/PS1dE9 mice
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17140703
DOI
10.1016/j.neurobiolaging.2006.10.029
PII: S0197-4580(06)00402-7
Knihovny.cz E-resources
- MeSH
- Acetylcholinesterase metabolism MeSH
- Amyloid beta-Protein Precursor genetics metabolism MeSH
- Analysis of Variance MeSH
- Animals, Genetically Modified MeSH
- Guanosine 5'-O-(3-Thiotriphosphate) metabolism MeSH
- Humans MeSH
- Mutation * MeSH
- Mice MeSH
- N-Methylscopolamine metabolism MeSH
- Synaptic Transmission genetics MeSH
- Piperidines metabolism pharmacology MeSH
- Presenilin-1 genetics MeSH
- Receptors, Muscarinic metabolism MeSH
- Protein Binding drug effects MeSH
- Age Factors MeSH
- Vesicular Acetylcholine Transport Proteins metabolism MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acetylcholinesterase MeSH
- Amyloid beta-Protein Precursor MeSH
- Guanosine 5'-O-(3-Thiotriphosphate) MeSH
- N-Methylscopolamine MeSH
- Piperidines MeSH
- Presenilin-1 MeSH
- Receptors, Muscarinic MeSH
- Slc18a3 protein, mouse MeSH Browser
- vesamicol MeSH Browser
- Vesicular Acetylcholine Transport Proteins MeSH
We assessed the integrity of cholinergic neurotransmission in parietal cortex of young adult (7 months) and aged (17 months) transgenic APPswe/PS1dE9 female mice compared to littermate controls. Choline acetyltransferase and acetylcholinesterase activity declined age-dependently in both genotypes, whereas both age- and genotype-dependent decline was found in butyrylcholinesterase activity, vesicular acetylcholine transporter density, muscarinic receptors and carbachol stimulated binding of GTP gamma S in membranes as a functional indicator of muscarinic receptor coupling to G-proteins. Notably, vesicular acetylcholine transporter levels and muscarinic receptor-G-protein coupling were impaired in transgenic mice already at the age of 7 months compared to wild type littermates. Thus, brain amyloid accumulation in this mouse model is accompanied by a serious deterioration of muscarinic transmission already before the mice manifest significant cognitive deficits.
Department of Neurochemistry Institute of Physiology CAS Vídenská 1083 14220 Prague 4 Czech Republic
References provided by Crossref.org
Apolipoprotein E4 reduces evoked hippocampal acetylcholine release in adult mice
Allosteric Modulation of Muscarinic Acetylcholine Receptors
