Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17176773
DOI
10.1007/bf02931597
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- Escherichia coli metabolismus MeSH
- herpesvirus 1 koní imunologie MeSH
- peptidová knihovna MeSH
- proteiny virového obalu imunologie metabolismus MeSH
- protilátky virové biosyntéza imunologie MeSH
- psi MeSH
- rekombinantní proteiny biosyntéza MeSH
- specificita protilátek MeSH
- variabilní oblast imunoglobulinu biosyntéza imunologie MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- glycoprotein D, Equid herpesvirus 1 MeSH Prohlížeč
- peptidová knihovna MeSH
- proteiny virového obalu MeSH
- protilátky virové MeSH
- rekombinantní proteiny MeSH
- variabilní oblast imunoglobulinu MeSH
Single chain Fv (scFv) molecules generated by phage-display technology represent a new and efficient tool in the research and diagnostics of infectious diseases. The recombinant glycoprotein D of Equid herpesvirus 1 was successfully expressed in E. coli cells. The protein was produced predominantly in soluble fraction and was then purified on a nickel-agarose column. The scFv antibodies against the glycoprotein were selected and several clones of glycoprotein D-specific scFv-antibodies were identified; t of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and used as reagent in an immunofluorescence test.
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