Determination of 17alpha-hydroxypregnenolone sulfate and its application in diagnostics
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17298837
DOI
10.1016/j.steroids.2006.11.026
PII: S0039-128X(06)00261-3
Knihovny.cz E-resources
- MeSH
- 17-alpha-Hydroxypregnenolone analogs & derivatives blood metabolism MeSH
- Child MeSH
- Adult MeSH
- Gestational Age MeSH
- Middle Aged MeSH
- Humans MeSH
- Menstrual Cycle MeSH
- Adolescent MeSH
- Child, Preschool MeSH
- Radioimmunoassay methods MeSH
- Reference Values MeSH
- Sex Factors MeSH
- Pregnancy MeSH
- Age Factors MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 17-alpha-Hydroxypregnenolone MeSH
- 17-hydroxypregnenolone sulfate MeSH Browser
New combined radioimmunoassay for determination of 17-hydroxypregnenolone sulfate (17-PregS) involving the hydrolysis of analyte by methanolysis was developed. 17-PregS, in addition to being secreted by the adrenals, is also formed by peripheral sulfoconjugation of 17-hydroxypregnenolone (17-Preg) or directly by hydroxylation of pregnenolone sulfate with 17alpha-hydroxylase/C17-20lyase. The measurement of 17-PregS can be used as a tool for detection of enzymatic deficiency particularly in pregnancy and for detection of congenital adrenal hyperplasia or gonadal dysfunction. The serum levels of 17-PregS, 17-Preg, dehydroepiandrosterone, dehydroepiandrosterone sulfate, pregnenolone and pregnenolone sulfate were measured in different age groups of human and in pregnant women respecting the age of gestation. The levels of 17-PregS are approximately three times higher than the levels of free 17-Preg in all subject groups. The levels of 17-PregS during pregnancy reached the local minimum in the 3rd month of gestation. The ratio of 17-PregS to free 17-Preg showed increasing profile during pregnancy with a maximum in the 8th month of gestation. These findings indicate that, the conversion of pregnenolone sulfate to 17-PregS is the major metabolic pathway for biosynthesis of 17-PregS.
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