Recognition of DNA three-way junctions by metallosupramolecular cylinders: gel electrophoresis studies
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA chemistry MeSH
- Electrophoresis, Polyacrylamide Gel methods MeSH
- Nucleic Acid Conformation * MeSH
- Metals chemistry MeSH
- Crystallography, X-Ray MeSH
- Models, Molecular MeSH
- Base Sequence MeSH
- Spectrophotometry, Ultraviolet MeSH
- Stereoisomerism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- Metals MeSH
The interaction of metallosupramolecular cylinders with DNA three-way junctions has been studied by gel electrophoresis. A recent X-ray crystal structure of a palindromic oligonucleotide forming part of a complex with such a cylinder revealed binding at the heart of a three-way junction structure. The studies reported herein confirm that this is not solely an artefact of crystallisation and reveal that this is a potentially very powerful new mode of DNA recognition with wide scope. The cylinders are much more effective at stabilizing three-way junctions than simple magnesium di-cations or organic or metallo-organic tetra-cations, with the M cylinder enantiomer being more effective than P. The recognition is not restricted to three-way junctions formed from palindromic DNA with a central AT step at the junction; non-palindromic three-way junctions and those with GC steps are also stabilised. The cylinder is also revealed to stabilise other Y-shaped junctions, such as that formed at a fraying point in duplex DNA (for example, a replication fork), and other DNA three-way junction structures, such as those containing unpaired nucleotides, perhaps by opening up this structure to access the central cavity.
References provided by Crossref.org
Metallohelices stabilize DNA three-way junctions and induce DNA damage in cancer cells
