HPLC method for determination of in vitro delivery through and into porcine skin of adefovir (PMEA)
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17400522
DOI
10.1016/j.jchromb.2007.03.012
PII: S1570-0232(07)00228-0
Knihovny.cz E-resources
- MeSH
- Adenine administration & dosage analogs & derivatives analysis pharmacokinetics MeSH
- Antiviral Agents administration & dosage analysis pharmacokinetics MeSH
- Administration, Cutaneous MeSH
- Skin Absorption MeSH
- Skin metabolism MeSH
- Organophosphonates administration & dosage analysis pharmacokinetics MeSH
- Swine MeSH
- Reproducibility of Results MeSH
- Spectrophotometry, Ultraviolet methods MeSH
- In Vitro Techniques MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- adefovir MeSH Browser
- Adenine MeSH
- Antiviral Agents MeSH
- Organophosphonates MeSH
A simple HPLC/UV method for the determination of the transdermal permeation and dermal penetration of a broad-spectrum antiviral drug adefovir (PMEA) was developed. The separation was achieved on a C18 column with the mobile phase composed of 10 mM KH2PO4 and 2 mM Bu4NHSO4 at pH 6.0 and 7% acetonitrile. The method was validated with respect to selectivity, linearity (0.1-50 microg/ml), precision, accuracy, and stability. Transdermal permeation of 2% PMEA was studied in vitro using the Franz diffusion cell and porcine skin. The flux values were 1.8, 3.0, and 0.6 microg/cm2/h from aqueous donor samples at pH 3.4 and 7.4, and isopropyl myristate, respectively. The respective skin concentrations at 48 h were 294, 263, and 971 microg/g from these vehicles. These results will serve as a lead for further studies on transdermal and topical delivery of antivirals from the group of acyclic nucleoside phosphonates.
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