Characterisation of Zygosaccharomyces rouxii centromeres and construction of first Z. rouxii centromeric vectors

. 2007 ; 15 (4) : 439-45. [epub] 20070515

Jazyk angličtina Země Nizozemsko Médium print-electronic

Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid17487563

Zygosaccharomyces rouxii is a hemiascomycetous yeast known for its high osmotolerance, the basis of which still remains unknown. By exploring the Génolevures I database, four Z. rouxii fragments homologous to Saccharomyces cerevisiae centromeres were identified. Two of them were subjected to further analysis. Their function as centromeres in Z. rouxii was proved, and they were localized to Z. rouxii chromosomes II and VII, respectively. The species-specificity of centromeres was observed; plasmids with a Z. rouxii centromere were not recognized as centromeric in S. cerevisiae, and a S. cerevisiae centromere did not function as a centromere in Z. rouxii. Constructed plasmids bearing Z. rouxii centromeres serve as the first specific centromeric plasmids, and thus contribute to the so-far limited set of genetic tools needed to study the Z. rouxii specific features.

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Gene. 1987;57(2-3):267-72 PubMed

Gene. 1985;33(1):103-19 PubMed

Cell. 1989 Jul 28;58(2):409-19 PubMed

Biotechnology (N Y). 1990 Mar;8(3):223-7 PubMed

Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11374-9 PubMed

Nature. 2004 Jul 1;430(6995):35-44 PubMed

Curr Opin Genet Dev. 1998 Apr;8(2):212-8 PubMed

Science. 1996 Oct 25;274(5287):546, 563-7 PubMed

Mol Gen Genet. 1993 Jan;236(2-3):355-62 PubMed

Mol Microbiol. 1990 Mar;4(3):329-36 PubMed

J Microbiol Methods. 2003 Nov;55(2):481-4 PubMed

FEBS Lett. 2000 Dec 22;487(1):3-12 PubMed

Gene. 1988 Dec 30;74(2):527-34 PubMed

Yeast. 2000 Nov;16(15):1377-85 PubMed

FEBS Lett. 2000 Dec 22;487(1):52-5 PubMed

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