Organization of specific genomic regions of Zygosaccharomyces rouxii and Pichia sorbitophila: comparison with Saccharomyces cerevisiae
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11054818
DOI
10.1002/1097-0061(200011)16:15<1377::aid-yea637>3.0.co;2-0
PII: 10.1002/1097-0061(200011)16:15<1377::AID-YEA637>3.0.CO;2-0
Knihovny.cz E-resources
- MeSH
- Chromosomes, Fungal chemistry genetics MeSH
- DNA, Fungal chemistry MeSH
- DNA Primers chemistry MeSH
- DNA Probes chemistry MeSH
- Genome, Fungal * MeSH
- Karyotyping MeSH
- Chromosome Mapping MeSH
- Molecular Sequence Data MeSH
- Pichia chemistry genetics MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Saccharomyces cerevisiae chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Blotting, Southern MeSH
- Zygosaccharomyces chemistry genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Fungal MeSH
- DNA Primers MeSH
- DNA Probes MeSH
The genomes of Zygosaccharomyces rouxii and Pichia sorbitophila were partially explored. The genome of Z. rouxii CBS 732 consists of seven chromosomes with an approximate size of 1.0-2.75 Mb, 12.8 Mb in total. Five of the chromosomes were labelled with specific probes. Three Z. rouxii genomic DNA fragments were sequenced; all 10 ORFs found were without introns and they have homologues in S. cerevisiae. Gene order comparison revealed that the organization is partially conserved in both species. The genome of P. sorbitophila CBS 7064 consists of seven chromosomes with an approximate size of 1.0-2.9 Mb, 13.9 Mb in total. Three of the chromosomes were labelled with specific probes. The sequencing of a 5.2 kb genomic DNA fragment revealed three ORFs, but no conservation of their organization was found, although all of them have their respective homologues in S. cerevisiae. According to our results, the presence of two overlapping ORFs in S. cerevisiae (YJL107c-YJL108c) could be interpreted as the result of a frameshift mutation.
References provided by Crossref.org
GENBANK
Y18559, Y18560, Y18561
