Organization of specific genomic regions of Zygosaccharomyces rouxii and Pichia sorbitophila: comparison with Saccharomyces cerevisiae
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
11054818
DOI
10.1002/1097-0061(200011)16:15<1377::aid-yea637>3.0.co;2-0
PII: 10.1002/1097-0061(200011)16:15<1377::AID-YEA637>3.0.CO;2-0
Knihovny.cz E-zdroje
- MeSH
- chromozomy hub chemie genetika MeSH
- DNA fungální chemie MeSH
- DNA primery chemie MeSH
- DNA sondy chemie MeSH
- genom fungální * MeSH
- karyotypizace MeSH
- mapování chromozomů MeSH
- molekulární sekvence - údaje MeSH
- Pichia chemie genetika MeSH
- pulzní gelová elektroforéza MeSH
- Saccharomyces cerevisiae chemie genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- Southernův blotting MeSH
- Zygosaccharomyces chemie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA fungální MeSH
- DNA primery MeSH
- DNA sondy MeSH
The genomes of Zygosaccharomyces rouxii and Pichia sorbitophila were partially explored. The genome of Z. rouxii CBS 732 consists of seven chromosomes with an approximate size of 1.0-2.75 Mb, 12.8 Mb in total. Five of the chromosomes were labelled with specific probes. Three Z. rouxii genomic DNA fragments were sequenced; all 10 ORFs found were without introns and they have homologues in S. cerevisiae. Gene order comparison revealed that the organization is partially conserved in both species. The genome of P. sorbitophila CBS 7064 consists of seven chromosomes with an approximate size of 1.0-2.9 Mb, 13.9 Mb in total. Three of the chromosomes were labelled with specific probes. The sequencing of a 5.2 kb genomic DNA fragment revealed three ORFs, but no conservation of their organization was found, although all of them have their respective homologues in S. cerevisiae. According to our results, the presence of two overlapping ORFs in S. cerevisiae (YJL107c-YJL108c) could be interpreted as the result of a frameshift mutation.
Citace poskytuje Crossref.org
GENBANK
Y18559, Y18560, Y18561
