The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24715459
DOI
10.1242/dev.105940
PII: 141/8/1694
Knihovny.cz E-zdroje
- Klíčová slova
- Centromere, Chromosome bridging, Mice, Nucleolus precursor body, Replication stress, Satellite DNA,
- MeSH
- blastocysta cytologie metabolismus MeSH
- buněčné jadérko metabolismus MeSH
- centromera metabolismus MeSH
- embryo savčí cytologie metabolismus MeSH
- genetická transkripce MeSH
- genom genetika MeSH
- heterochromatin genetika MeSH
- messenger RNA genetika metabolismus MeSH
- mikrosatelitní repetice genetika MeSH
- minisatelitní repetice genetika MeSH
- myši MeSH
- oocyty cytologie metabolismus MeSH
- posttranskripční úpravy RNA genetika MeSH
- prekurzory RNA genetika MeSH
- rekombinace genetická genetika MeSH
- replikace DNA genetika MeSH
- restrukturace chromatinu genetika MeSH
- RNA ribozomální biosyntéza genetika MeSH
- S fáze genetika MeSH
- savčí chromozomy metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- heterochromatin MeSH
- messenger RNA MeSH
- prekurzory RNA MeSH
- RNA ribozomální MeSH
The oocyte (maternal) nucleolus is essential for early embryonic development and embryos originating from enucleolated oocytes arrest at the 2-cell stage. The reason for this is unclear. Surprisingly, RNA polymerase I activity in nucleolus-less mouse embryos, as manifested by pre-rRNA synthesis, and pre-rRNA processing are not affected, indicating an unusual role of the nucleolus. We report here that the maternal nucleolus is indispensable for the regulation of major and minor satellite repeats soon after fertilisation. During the first embryonic cell cycle, absence of the nucleolus causes a significant reduction in major and minor satellite DNA by 12% and 18%, respectively. The expression of satellite transcripts is also affected, being reduced by more than half. Moreover, extensive chromosome bridging of the major and minor satellite sequences was observed during the first mitosis. Finally, we show that the absence of the maternal nucleolus alters S-phase dynamics and causes abnormal deposition of the H3.3 histone chaperone DAXX in pronuclei of nucleolus-less zygotes.
Citace poskytuje Crossref.org
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