Effect of cannabinoids on platelet serotonin uptake
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17508987
DOI
10.1111/j.1369-1600.2007.00065.x
PII: ADB065
Knihovny.cz E-resources
- MeSH
- Benzoxazines pharmacology MeSH
- Cell Membrane drug effects MeSH
- Adult MeSH
- Endocannabinoids MeSH
- Imipramine pharmacology MeSH
- Cannabinoids pharmacology MeSH
- Cocaine pharmacology MeSH
- Marijuana Smoking blood MeSH
- Arachidonic Acids pharmacology MeSH
- Humans MeSH
- Serotonin Plasma Membrane Transport Proteins drug effects MeSH
- Morpholines pharmacology MeSH
- Naphthalenes pharmacology MeSH
- Sex Characteristics MeSH
- Polyunsaturated Alkamides pharmacology MeSH
- Serotonin blood MeSH
- In Vitro Techniques MeSH
- Dronabinol pharmacology MeSH
- Blood Platelets drug effects MeSH
- Viscosity MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- (3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone MeSH Browser
- anandamide MeSH Browser
- Benzoxazines MeSH
- Endocannabinoids MeSH
- Imipramine MeSH
- Cannabinoids MeSH
- Cocaine MeSH
- Arachidonic Acids MeSH
- Serotonin Plasma Membrane Transport Proteins MeSH
- Morpholines MeSH
- Naphthalenes MeSH
- Polyunsaturated Alkamides MeSH
- Serotonin MeSH
- Dronabinol MeSH
Serotonin is involved in many of the same processes affected by cannabinoids; therefore, we investigated in vitro and in vivo effects of these drugs on the function of serotonin transporter. The effect of Delta(9)-tetrahydrocannabinol (Delta(9)-THC), endocannabinoid anandamide and synthetic cannabinoid receptor agonist WIN 55,212-2 on platelet serotonin uptake and membrane microviscosity was examined in 19 marijuana smokers and 20 controls. (1) Serotonin uptake was inhibited at higher doses of Delta(9)-THC (IC(50) = 139 micromol/l), anandamide (IC(50) = 201 micromol/l) or WIN 55,212-2 (IC(50) = 17.4 micromol/l); the inhibition was found non-competitive. Delta(9)-THC, anandamide and WIN 55,212-2 produced different effects on the membrane microviscosity. (2) Maximal velocity of platelet serotonin uptake was significantly increased in a group of chronic marijuana smokers suffering impairment of cognitive functions when compared with controls. Opposite effect of marijuana smoking on the serotonin uptake efficiency was observed in males beside females. In summary, this study provides evidence that (1) Activity of serotonin transporter is acutely affected by cannabinoids at relatively high drug concentrations; this effect is indirect and can be partially accounted for the changes in the membrane microviscosity. (2) Increase of maximal velocity of the serotonin uptake could be understood as adaptation change in the serotonergic system induced by chronic cannabis use. A hypothesis was supported that lowered serotonin uptake may reflect a gender-related differences in effects of psychoactive cannabinoids.
References provided by Crossref.org
Inhibition of monoamine oxidase activity by cannabinoids
