Adipokinetic hormone-induced enhancement of antioxidant capacity of Pyrrhocoris apterus hemolymph in response to oxidative stress
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17512258
DOI
10.1016/j.cbpc.2007.04.005
PII: S1532-0456(07)00109-3
Knihovny.cz E-resources
- MeSH
- Antioxidants metabolism pharmacology MeSH
- Glutathione metabolism MeSH
- Hemolymph drug effects metabolism MeSH
- Herbicides pharmacology MeSH
- Heteroptera drug effects metabolism MeSH
- Insect Hormones metabolism pharmacology MeSH
- Drug Therapy, Combination MeSH
- Pyrrolidonecarboxylic Acid analogs & derivatives metabolism pharmacology MeSH
- Brain drug effects metabolism MeSH
- Oligopeptides metabolism pharmacology MeSH
- Oxidative Stress drug effects physiology MeSH
- Paraquat pharmacology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- adipokinetic hormone MeSH Browser
- Antioxidants MeSH
- Glutathione MeSH
- Herbicides MeSH
- Insect Hormones MeSH
- Pyrrolidonecarboxylic Acid MeSH
- Oligopeptides MeSH
- Paraquat MeSH
The in vivo effects of oxidative stress on adipokinetic hormone (AKH) titer in short-winged (brachypterous) males of the firebug Pyrrhocoris apterus were tested using paraquat (PQ), a bipyridilium herbicide. PQ undergoes a cyclic redox reaction with oxygen during microsomal and electron transfer reactions forming free radicals in the insect body. Oxidative insult (40 pmol PQ) resulted in enhanced protein carbonylation (a biomarker for oxidative stress) and a depletion of glutathione (GSH) pool in the hemolymph. Interestingly, AKH titer was significantly enhanced in hemolymph at 4 h post inoculation of PQ, while its content in CNS (brain with corpora cardiaca) showed non-specific changes in comparable period. Co-injection of AKH with PQ (40 pmol each) reversed these effects by decreasing protein carbonyl formation, increasing reduced GSH levels, and enhancing the total antioxidant capacity of cell free plasma. Our results indicate that there is a positive feedback regulation between an oxidative stressor action and the level of AKH in insect body, and that AKHs might be involved in the activation of antioxidant protection mechanism.
Institute of Entomology Academy of Sciences Branisovská 31 CZ 370 05 Ceské Budejovice Czech Republic
References provided by Crossref.org
Insect Body Defence Reactions against Bee Venom: Do Adipokinetic Hormones Play a Role?
Hormonal Regulation of Response to Oxidative Stress in Insects-An Update
