Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
17548116
DOI
10.1016/j.jviromet.2007.04.010
PII: S0166-0934(07)00161-9
Knihovny.cz E-resources
- MeSH
- Ticks virology MeSH
- Encephalitis, Tick-Borne epidemiology virology MeSH
- Humans MeSH
- Molecular Epidemiology MeSH
- Reverse Transcriptase Polymerase Chain Reaction methods MeSH
- RNA, Viral isolation & purification MeSH
- Sensitivity and Specificity MeSH
- Encephalitis Viruses, Tick-Borne classification isolation & purification MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Viral MeSH
Tick-borne encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of tick-borne encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of tick-borne encephalitis virus.
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