Postnatal development of bacterial population in the gastrointestinal tract of calves
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17571805
DOI
10.1007/bf02932147
Knihovny.cz E-resources
- MeSH
- Bacteria classification genetics growth & development isolation & purification MeSH
- Bifidobacterium genetics growth & development isolation & purification MeSH
- DNA, Bacterial analysis MeSH
- Ecosystem MeSH
- Electrophoresis methods MeSH
- Escherichia coli genetics growth & development isolation & purification MeSH
- Feces microbiology MeSH
- Gastrointestinal Tract microbiology MeSH
- Animals, Newborn * MeSH
- Polymerase Chain Reaction methods MeSH
- DNA, Ribosomal analysis MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cattle * MeSH
- Animals MeSH
- Check Tag
- Cattle * MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
Bacterial 16S rDNA from fecal samples of two calves were amplified by PCR and analyzed by denaturing gradient gel electrophoresis; selected bands were sequenced. Escherichia coli and Bifidobacterium animalis were the initial colonizers, followed by species closely related to the genera Bacteroides, Clostridium and Faecalibacterium. Change of diet was connected with shifts of bacterial population and with the occurrence of many bacterial species that have not been cultured up to now. The diet change corresponded with an alteration in a volatile-fatty-acid concentration in fecal samples.
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