Photoperiodic and food signals control expression pattern of the clock gene, period, in the linden bug, Pyrrhocoris apterus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17660450
DOI
10.1177/0748730407303624
PII: 22/4/335
Knihovny.cz E-resources
- MeSH
- Period Circadian Proteins MeSH
- Photoperiod * MeSH
- Heteroptera genetics physiology MeSH
- Starvation MeSH
- Insect Proteins genetics MeSH
- Nuclear Proteins genetics MeSH
- RNA, Messenger metabolism MeSH
- Feeding Behavior MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Period Circadian Proteins MeSH
- Insect Proteins MeSH
- Nuclear Proteins MeSH
- RNA, Messenger MeSH
The temporal expression pattern of the circadian clock gene period was compared between heads of the linden bug, Pyrrhocoris apterus , kept under diapause-promoting short days (SD) and diapause-preventing long days (LD) using a real-time PCR quantification. Diapause or reproduction was programmed by photoperiod during the larval stage, but the first difference in per mRNA abundance between SD and LD insects was observed only after adult ecdysis. The expression level of per mRNA was markedly higher, up to more than 10-fold, in the destined-to diapause animals compared with those scheduled for reproduction. Up-regulation of per transcript was restricted to an early diapause peak, with the maximum expression on days 3 to 5 after adult ecdysis. Starvation reduced the peak level of per mRNA to about 50% of the value found in feeding females in the SD conditions, but per mRNA abundance was similarly low in fasting and feeding females in LD. Photoperiodic refractoriness in either wild-type postdiapause adults or in a selected nondiapause variant of P. apterus was associated with reproduction and low, LD-like levels of per mRNA under both SD and LD. Overall, the data suggest that the photoperiodic programming itself has no direct effect on per mRNA abundance, but it does determine the response of per transcript to food signals during subsequent expression of diapause/reproduction physiology.
References provided by Crossref.org
Light and Temperature Synchronizes Locomotor Activity in the Linden Bug, Pyrrhocoris apterus
