The thermal treatment effects on bovine blood neutrophil granulocytes apoptosis and necrosis in vitro
Language English Country Slovakia Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
17660586
Knihovny.cz E-resources
- MeSH
- Apoptosis * MeSH
- Radiation Injuries, Experimental MeSH
- Cryopreservation methods veterinary MeSH
- Necrosis * pathology MeSH
- Neutrophils pathology MeSH
- Leukocyte Count MeSH
- Flow Cytometry MeSH
- Cattle MeSH
- Temperature * MeSH
- Microscopy, Electron, Transmission MeSH
- Cell Survival MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
The aim of the study was to evaluate the effect of selected temperatures on viability (apoptosis and necrosis) of bovine blood neutrophil granulocytes (neutrophils) in vitro. The following temperatures were tested: -80, -20, 4, 23, 37 degrees C. Heparinised bovine blood was incubated for 1, 4 and 24 h under respective temperature. Apoptosis and necrosis of neutrophils were detected by light microscopy, transmission electron microscopy (TEM) and flow cytometry (FCM). From selected temperatures, 4 degrees C impaired the neutrophil viability least. The proportion of apoptotic and necrotic neutrophils amounted to (mean +/- SD) 5.25 +/- 3.53% and 0.83 +/- 0.38%; 7.09 +/- 2.07% and 1.64 +/- 0.50%; 35.39 +/- 12.53% and 5.46 +/- 1.46%; after 1, 4 and 24 h incubation, respectively. The temperature (4 degrees C) is the best alternative for short-term storage.
