The thermal treatment effects on bovine blood neutrophil granulocytes apoptosis and necrosis in vitro
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
17660586
Knihovny.cz E-zdroje
- MeSH
- apoptóza * MeSH
- experimentální radiační poranění MeSH
- kryoprezervace metody veterinární MeSH
- nekróza * patologie MeSH
- neutrofily patologie MeSH
- počet leukocytů MeSH
- průtoková cytometrie MeSH
- skot MeSH
- teplota * MeSH
- transmisní elektronová mikroskopie MeSH
- viabilita buněk MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
The aim of the study was to evaluate the effect of selected temperatures on viability (apoptosis and necrosis) of bovine blood neutrophil granulocytes (neutrophils) in vitro. The following temperatures were tested: -80, -20, 4, 23, 37 degrees C. Heparinised bovine blood was incubated for 1, 4 and 24 h under respective temperature. Apoptosis and necrosis of neutrophils were detected by light microscopy, transmission electron microscopy (TEM) and flow cytometry (FCM). From selected temperatures, 4 degrees C impaired the neutrophil viability least. The proportion of apoptotic and necrotic neutrophils amounted to (mean +/- SD) 5.25 +/- 3.53% and 0.83 +/- 0.38%; 7.09 +/- 2.07% and 1.64 +/- 0.50%; 35.39 +/- 12.53% and 5.46 +/- 1.46%; after 1, 4 and 24 h incubation, respectively. The temperature (4 degrees C) is the best alternative for short-term storage.
