Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17673300
DOI
10.1016/j.vetimm.2007.06.022
PII: S0165-2427(07)00215-2
Knihovny.cz E-zdroje
- MeSH
- B-lymfocyty imunologie MeSH
- CD antigeny imunologie MeSH
- imunofenotypizace MeSH
- lidé MeSH
- monoklonální protilátky imunologie MeSH
- prasata imunologie MeSH
- průtoková cytometrie MeSH
- psi imunologie MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- psi imunologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CD antigeny MeSH
- monoklonální protilátky MeSH
We have characterized a panel of commercially available anti-human monoclonal antibodies (mAbs) suitable for B-cell identification in pigs and dogs. The specificities of the mAbs were against CD20, CD21, CD22, and CD86. In addition to HM57, originally raised against human CD79alpha the broad cross-reactivity of which was documented more than 10 years ago, we recommend here a panel of several other mAbs as a useful tool for immunophenotyping and multicolor flow cytometry of canine and porcine B-lymphocytes. All six investigated antibodies did bind weakly to either canine or porcine lymphocytes (or both), but considerable weaker than for the human control cells. Four of them did bind to canine or porcine spleen section in immunohistochemistry. Monoclonal antibody against CD22 (clone RFB-4) was the only antibody in the tested panel the cross-reactivity of which was confirmed by Western blot. The advantages and limits of cross-reactive mAbs in studies on animal B-cells are discussed.
Citace poskytuje Crossref.org
The ontogeny of the porcine immune system
