Dynamism in physiology and gene transcription during reproductive diapause in a heteropteran bug, Pyrrhocoris apterus
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
17880995
DOI
10.1016/j.jinsphys.2007.08.004
PII: S0022-1910(07)00189-8
Knihovny.cz E-zdroje
- MeSH
- biologické hodiny fyziologie MeSH
- DNA primery genetika MeSH
- energetický metabolismus fyziologie MeSH
- fotoperioda MeSH
- fyziologická adaptace fyziologie MeSH
- genetická transkripce fyziologie MeSH
- Heteroptera růst a vývoj metabolismus fyziologie MeSH
- klonování DNA MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- rozmnožování fyziologie MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- stadia vývoje fyziologie MeSH
- teplota MeSH
- vývojová regulace genové exprese * MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA primery MeSH
Reproductive diapause was characterized in females of Pyrrhocoris apterus using physiological parameters (diapause intensity, photoperiodic responsiveness, oxygen consumption, mass and hydration) and changes in relative abundance of mRNA transcripts of eight different genes coding for proteins implemented in energy metabolism, cryoprotectant biosynthesis, biological clocks, and hormonal receptors. Changes in diapause intensity served as a basis for distinguishing successive phases of diapause development, which were driven both endogenously (under constant environmental conditions) and exogenously (in response to a change in environmental conditions). Changes in the relative levels of transcripts of genes coding for aldose reductase (AR) and sorbitol dehydrogenase (SoDH) closely matched those of diapause intensity and thus appeared as promising molecular markers of diapause and its development. During the initiation phase, the intensity of diapause and the levels of AR and SoDH transcripts increased and reached a maximum. During maintenance, under a constant temperature of 20 degrees C and short-day photoperiod, the intensity of diapause and the levels of both transcripts first decreased and, later, were maintained constant. Termination of diapause was stimulated by cold, during which the intensity of diapause and the levels of both transcripts further decreased. Upon resumption of direct development (oogenesis, mating and oviposition), the relative abundances of AR and SoDH transcripts decreased to trace levels.
Citace poskytuje Crossref.org
Light and Temperature Synchronizes Locomotor Activity in the Linden Bug, Pyrrhocoris apterus
