Dynamism in physiology and gene transcription during reproductive diapause in a heteropteran bug, Pyrrhocoris apterus
Language English Country Great Britain, England Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
17880995
DOI
10.1016/j.jinsphys.2007.08.004
PII: S0022-1910(07)00189-8
Knihovny.cz E-resources
- MeSH
- Biological Clocks physiology MeSH
- DNA Primers genetics MeSH
- Energy Metabolism physiology MeSH
- Photoperiod MeSH
- Adaptation, Physiological physiology MeSH
- Transcription, Genetic physiology MeSH
- Heteroptera growth & development metabolism physiology MeSH
- Cloning, Molecular MeSH
- Molecular Sequence Data MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Reproduction physiology MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Life Cycle Stages physiology MeSH
- Temperature MeSH
- Gene Expression Regulation, Developmental * MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA Primers MeSH
Reproductive diapause was characterized in females of Pyrrhocoris apterus using physiological parameters (diapause intensity, photoperiodic responsiveness, oxygen consumption, mass and hydration) and changes in relative abundance of mRNA transcripts of eight different genes coding for proteins implemented in energy metabolism, cryoprotectant biosynthesis, biological clocks, and hormonal receptors. Changes in diapause intensity served as a basis for distinguishing successive phases of diapause development, which were driven both endogenously (under constant environmental conditions) and exogenously (in response to a change in environmental conditions). Changes in the relative levels of transcripts of genes coding for aldose reductase (AR) and sorbitol dehydrogenase (SoDH) closely matched those of diapause intensity and thus appeared as promising molecular markers of diapause and its development. During the initiation phase, the intensity of diapause and the levels of AR and SoDH transcripts increased and reached a maximum. During maintenance, under a constant temperature of 20 degrees C and short-day photoperiod, the intensity of diapause and the levels of both transcripts first decreased and, later, were maintained constant. Termination of diapause was stimulated by cold, during which the intensity of diapause and the levels of both transcripts further decreased. Upon resumption of direct development (oogenesis, mating and oviposition), the relative abundances of AR and SoDH transcripts decreased to trace levels.
References provided by Crossref.org
Light and Temperature Synchronizes Locomotor Activity in the Linden Bug, Pyrrhocoris apterus
