Functional expression of amine oxidase from Aspergillus niger (AO-I) in Saccharomyces cerevisiae
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- aminy metabolismus MeSH
- Aspergillus niger enzymologie genetika MeSH
- chromatografie DEAE-celulózová MeSH
- fungální proteiny chemie genetika metabolismus MeSH
- histaminasa chemie genetika metabolismus MeSH
- hmotnostní spektrometrie MeSH
- kinetika MeSH
- klonování DNA MeSH
- molekulární sekvence - údaje MeSH
- rekombinantní proteiny chemie genetika metabolismus MeSH
- Saccharomyces cerevisiae genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční seřazení MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aminy MeSH
- fungální proteiny MeSH
- hexylamine MeSH Prohlížeč
- histaminasa MeSH
- rekombinantní proteiny MeSH
The aim of this work was to prepare recombinant amine oxidase from Aspergillus niger after overexpressing in yeast. The yeast expression vector pDR197 that includes a constitutive PMA1 promoter was used for the expression in Saccharomyces cerevisiae. Recombinant amine oxidase was extracted from the growth medium of the yeast, purified to homogeneity and identified by activity assay and MALDI-TOF peptide mass fingerprinting. Similarity search in the newly published A. niger genome identified six genes coding for copper amine oxidase, two of them corresponding to the previously described enzymes AO-I a methylamine oxidase and three other genes coding for FAD amine oxidases. Thus, A. niger possesses an enormous metabolic gear to grow on amine compounds and thus support its saprophytic lifestyle.
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