- MeSH
- antagonisté histaminu H1 farmakologie terapeutické užití MeSH
- biogenní aminy imunologie metabolismus škodlivé účinky MeSH
- časná přecitlivělost MeSH
- histamin * imunologie metabolismus škodlivé účinky MeSH
- histaminasa metabolismus terapeutické užití účinky léků MeSH
- lidé středního věku MeSH
- lidé MeSH
- potravinová alergie etiologie farmakoterapie prevence a kontrola MeSH
- tenké střevo metabolismus mikrobiologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The N-glycosylation in pea seedling amine oxidase and lentil seedling amine oxidase was analyzed in the present work. For that purpose, the enzymes were purified as native proteins from their natural sources. An enzymatic deglycosylation of pea seedling amine oxidase by endoglycosidase H under denaturing conditions combined with its proteolytic digestion by trypsin was carried out in order to analyze both N-glycans and "trimmed" N-glycopeptides with a residual N-acetylglucosamine attached at the originally occupied N-glycosylation sites. The released N-glycans were subjected to a manual chromatographic purification followed by MALDI-TOF/TOF MS. MS and MS/MS analyses were also performed directly on peptides and N-glycopeptides generated by proteolytic digestion of the studied enzymes. Sequencing of glycopeptides by MALDI-TOF/TOF MS/MS after their separation on a RP using a microgradient chromatographic device clearly demonstrated binding of paucimannose and hybrid N-glycan structures at Asn558. Such carbohydrates have been reported to exist in many plant N-glycoproteins, e.g. in peroxidases. Although high-mannose glycan structures were identified after the enzymatic deglycosylation, they could not be assigned to a particular N-glycosylation site. The presence of unoccupied glycosylation sites in several peptides was also confirmed from MS/MS results.
- MeSH
- glykopeptidy analýza chemie izolace a purifikace MeSH
- glykosylace MeSH
- histaminasa analýza chemie metabolismus MeSH
- Lathyrus chemie enzymologie MeSH
- mannosyl-glykoprotein endo-beta-N-acetylglukosaminidasa chemie MeSH
- molekulární modely MeSH
- molekulární sekvence - údaje MeSH
- polysacharidy analýza chemie izolace a purifikace MeSH
- rostlinné proteiny analýza chemie metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční seřazení MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Histamínová intolerancia (HIT) je patologický proces, pri ktorom následkom nepomeru medzi prísunom histamínu a schopnosťou organizmu odbúrať ho vznikajú histamínom mediované nežiaduce reakcie. Napriek mnohým poznatkom, ktoré o histamínovej intolerancii máme, je toto ochorenie aj v dnešnej dobe málo a nie príliš úspešne diagnostikované, pretože sa prejavuje veľmi rôznorodými klinickými symptómami, ktoré bývajú často nesprávne interpretované, či už samotným pacientom, alebo dokonca aj lekárom. Klinické symptómy a ich provokácia určitými druhmi jedál, nápojov alebo liekov bývajú často pripisované iným typom ochorení, ako napríklad potravinovým alergiám, intolerancii sulfitov alebo iných biogénnych amínov (napr. tyramínu), mastocytóze, psychosomatickým ochoreniam či nežiaducim reakciám vyvolaným liečivami. Správna diagnostika tohto ochorenia a následná terapia, založená na úprave diéty s vynechaním potravín bohatých na histamín a s uplementácii diaminooxidázy, môže viesť k zlepšeniu kvality života pacientov trpiacich HIT.
Histamine intolerance (HIT) is a pathological process that results from a disbalance between levels of released histamine and t he ability of the body to metabolize it. Accumulated histamine leads to the onset of “histamine mediated” reactions which are usually excessive and decrease quality of life. Although we have a lot of knowledge about histamine intolerance, HIT is still vastly underestimated, because it manifests via the diversity of clinical symptoms, that are often misinterpreted by the patient and sometimes even by a physician. Clinical symptoms and their provocation by certain kinds of food, beverages and drugs are often attributed to the different diseases, such as food allergy and intolerance of sulfites, or other biogenic amines (eg. tyramine), mastocytosis, psychosomatic diseases or adverse drug reactions in general. P roper diagnosis of HIT followed by therapy based on histamine – free diet and supplementation of diaminooxidase can considerably improve patient‘s quality of life.
- MeSH
- antihistaminika terapeutické užití MeSH
- bolesti hlavy etiologie MeSH
- histamin-N-methyltransferasa krev metabolismus nedostatek MeSH
- histamin * aplikace a dávkování metabolismus škodlivé účinky MeSH
- histaminasa krev metabolismus nedostatek terapeutické užití MeSH
- kožní manifestace MeSH
- lidé MeSH
- potravinová alergie diagnóza dietoterapie etiologie MeSH
- příznaky a symptomy ústrojí dýchacího MeSH
- příznaky a symptomy ústrojí trávicího MeSH
- uvolňování histaminu * fyziologie imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Uric acid is involved in nitrogenous waste in animals, together with ammonia and urea. Uric acid has also antioxidant properties and is a surrogate marker of metabolic syndrome. We observed that the elevated plasma uric acid of high-fat fed mice was normalized by benzylamine treatment. Indeed, benzylamine is the reference substrate of semicarbazide-sensitive amine oxidase (SSAO), an enzyme highly expressed in fat depots and vessels, which generates ammonia when catalysing oxidative deamination. Ammonia interferes with uric acid metabolism/solubility. Our aim was therefore to investigate whether the lowering action of benzylamine on uric acid was related to an improvement of diabetic complications, or was connected with SSAO-dependent ammonia production. First, we observed that benzylamine administration lowered plasma uric acid in diabetic db/db mice while it did not modify uric acid levels in normoglycemic and lean mice. In parallel, benzylamine improved the glycemic control in diabetic but not in normoglycemic mice, while plasma urea remained unaltered. Then, uric acid plasma levels were measured in mice invalidated for AOC3 gene, encoding for SSAO. These mice were unable to oxidize benzylamine but were not diabetic and exhibited unaltered plasma uric levels. Therefore, activated or abolished ammonia production by SSAO was without influence on uric acid in the context of normoglycemia. Our observations confirm that plasma uric acid increases with diabetes and can be normalized when glucose tolerance is improved. They also show that uric acid, a multifunctional metabolite at the crossroads of nitrogen waste and of antioxidant defences, can be influenced by SSAO, in a manner apparently related to changes in glucose homeostasis.
- MeSH
- aktivace enzymů MeSH
- aktivátory enzymů farmakologie MeSH
- amoniak metabolismus MeSH
- benzylaminy farmakologie MeSH
- časové faktory MeSH
- diabetes mellitus enzymologie farmakoterapie krev MeSH
- down regulace MeSH
- histaminasa genetika metabolismus nedostatek MeSH
- hyperurikemie enzymologie farmakoterapie krev MeSH
- hypoglykemika farmakologie MeSH
- krevní glukóza metabolismus účinky léků MeSH
- kyselina močová krev MeSH
- modely nemocí na zvířatech MeSH
- molekuly buněčné adheze genetika metabolismus nedostatek MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- arteriae mesentericae enzymologie MeSH
- diabetes mellitus 2. typu enzymologie MeSH
- financování organizované MeSH
- histaminasa analýza metabolismus MeSH
- kinetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- monoaminoxidasa analýza metabolismus MeSH
- noradrenalin metabolismus MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
This review reports on inhibitors of copper-containing amine oxidases and flavoprotein polyamine oxidases, which are structurally based on diamines. In the introduction, basic characteristics and classification of amine oxidases are described together with the significance of their synthetic inhibitors. The following text is divided into several chapters, which deal with diaminoketones, aza-diamines, unsaturated diamine analogs and diamines with heterocyclic substituents. Then it continues with diamine- and agmatine-based inhibitors of polyamine oxidases. Each chapter gives detailed information on the inhibition mode, potency and structural relationships. The conclusion points out possible roles of mechanism-based inhibitors of amine oxidases in physiological and medicinal research.
- MeSH
- agmatin farmakologie chemie MeSH
- aktivace enzymů fyziologie účinky léků MeSH
- diaminy farmakologie chemie MeSH
- financování organizované MeSH
- histaminasa antagonisté a inhibitory metabolismus MeSH
- inhibitory enzymů farmakologie chemie MeSH
- ketony farmakologie chemie MeSH
- lidé MeSH
- molekulární struktura MeSH
- oxidoreduktasy působící na CH-NH vazby antagonisté a inhibitory metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
