Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
17953628
DOI
10.1111/j.1365-2265.2007.02944.x
PII: CEN2944
Knihovny.cz E-resources
- MeSH
- Adiponectin blood genetics MeSH
- Antigens, Differentiation, Myelomonocytic genetics MeSH
- C-Reactive Protein analysis MeSH
- Antigens, CD genetics MeSH
- Adult MeSH
- Gene Expression MeSH
- Adaptation, Physiological MeSH
- Interleukin-6 genetics MeSH
- Insulin blood MeSH
- Blood Glucose analysis MeSH
- Receptors, Leptin blood MeSH
- Humans MeSH
- Anorexia Nervosa immunology metabolism MeSH
- RNA, Messenger analysis MeSH
- Statistics, Nonparametric MeSH
- Paracrine Communication * MeSH
- Subcutaneous Fat chemistry immunology metabolism MeSH
- Malnutrition immunology metabolism MeSH
- Resistin blood genetics MeSH
- Case-Control Studies MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Adiponectin MeSH
- Antigens, Differentiation, Myelomonocytic MeSH
- C-Reactive Protein MeSH
- Antigens, CD MeSH
- CD68 antigen, human MeSH Browser
- Interleukin-6 MeSH
- Insulin MeSH
- Blood Glucose MeSH
- Receptors, Leptin MeSH
- RNA, Messenger MeSH
- Resistin MeSH
OBJECTIVE: To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function of adipose tissue on both circulating and subcutaneous fat mRNA expression level. PATIENTS AND DESIGN: A total of 12 patients with anorexia nervosa and 18 normal weight age-matched women underwent anthropometric examination, single blood drawing and subcutaneous adipose tissue biopsy. MEASUREMENTS: Serum concentrations of high-sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin-6 and insulin were measured by Luminex, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits. Subcutaneous adipose tissue mRNA expression of the same adipokines, adiponectin receptors 1 and 2 and immunocompetent cells marker CD68 was measured by real-time polymerase chain reaction (PCR). RESULTS: Decreased body fat content of patients with anorexia nervosa was accompanied by reduced hsCRP, leptin and increased adiponectin and soluble leptin receptor. Resistin, interleukin-6 and insulin levels did not differ from those of the control group. Fat mRNA adiponectin, leptin, interleukin-6 and CD68 expression was reduced, resistin mRNA expression was increased and adiponectin receptor 1 and 2 expression were unchanged as compared to the control group. CONCLUSIONS: Local perturbations in resistin, adiponectin and interleukin-6 mRNA expression in subcutaneous adipose tissue are not reflected by its circulating levels. These changes could be involved in some local metabolic disturbances in subcutaneous adipose tissue of anorexia nervosa patients.
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