Alterations in mitochondrial morphology of Schizosaccharomyces pombe induced by cell-death promoting agents
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18062187
DOI
10.1007/bf02932093
Knihovny.cz E-zdroje
- MeSH
- buněčná smrt účinky léků fyziologie MeSH
- DNA fungální genetika MeSH
- fluorescenční mikroskopie MeSH
- karbocyaniny chemie MeSH
- kyselina octová farmakologie MeSH
- mitochondriální DNA genetika MeSH
- mitochondrie účinky léků fyziologie ultrastruktura MeSH
- plazmidy genetika MeSH
- protein X asociovaný s bcl-2 biosyntéza genetika fyziologie MeSH
- Schizosaccharomyces účinky léků genetika fyziologie MeSH
- Southernův blotting MeSH
- transformace genetická MeSH
- zelené fluorescenční proteiny biosyntéza genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 3,3'-dihexyl-2,2'-oxacarbocyanine MeSH Prohlížeč
- DNA fungální MeSH
- karbocyaniny MeSH
- kyselina octová MeSH
- mitochondriální DNA MeSH
- protein X asociovaný s bcl-2 MeSH
- zelené fluorescenční proteiny MeSH
The effect of the yeast cell-death inducing agents, Bax and acetic acid, on mitochondrial structure of Schizosaccharomyces pombe was studied. Comparison of mitochondrial structures in cells grown on different substrates and visualized with different probes revealed variations in their morphology. Cells grown on respiratory C sources as well as in the presence of antimycin A exhibited punctuated mitochondria when visualized with mitochondrially targeted green fluorescent protein, while they still appeared as tubular structures when stained with DiOC6(3). Both expression of Bax and acetic acid treatment induced fragmentation and aggregation of mitochondrial network, which could be prevented by coexpression of Bcl-XL. Aberrant mitochondrial morphology generated by either Bax or acetic acid was not accompanied with the loss of mitochondrial genome (mtDNA), indicating that alterations of mitochondrial morphology following death stimuli follow different mechanisms than those involved in mitochondrial inheritance mutants.
Zobrazit více v PubMed
J Biol Chem. 1990 Jul 5;265(19):10857-64 PubMed
J Bacteriol. 2000 Dec;182(24):6933-9 PubMed
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11664-8 PubMed
Biochim Biophys Acta. 1999 Mar 9;1410(3):229-36 PubMed
Yeast. 1992 Nov;8(11):923-33 PubMed
Nat Cell Biol. 1999 Oct;1(6):E149-50 PubMed
Mol Cell Biol. 1996 Nov;16(11):6494-508 PubMed
Trends Cell Biol. 2005 Apr;15(4):179-83 PubMed
Mol Biol Cell. 2004 Sep;15(9):3994-4002 PubMed
Curr Opin Microbiol. 2004 Dec;7(6):655-60 PubMed
Trends Biochem Sci. 2001 Jan;26(1):23-9 PubMed
J Cell Biol. 2002 Dec 23;159(6):931-8 PubMed
Mol Biol Cell. 1997 Feb;8(2):325-39 PubMed
Methods Enzymol. 1991;194:795-823 PubMed
Dev Cell. 2001 Oct;1(4):515-25 PubMed
Genes Dev. 2004 Nov 15;18(22):2785-97 PubMed
Nat Genet. 2001 Jul;28(3):272-5 PubMed
Nucleic Acids Res. 1993 Jun 25;21(12):2955-6 PubMed
Curr Opin Cell Biol. 2005 Aug;17(4):384-8 PubMed
Mol Biol Cell. 1997 Jul;8(7):1233-42 PubMed
Genes Dev. 1992 Mar;6(3):380-9 PubMed
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9238-42 PubMed
J Biol Chem. 1998 Aug 7;273(32):20150-5 PubMed
Curr Opin Cell Biol. 2003 Aug;15(4):482-8 PubMed
Biochem Biophys Res Commun. 1998 Oct 29;251(3):720-6 PubMed
Anal Biochem. 1977 Jan;77(1):110-21 PubMed
FEMS Yeast Res. 2004 Nov;5(2):149-56 PubMed
Annu Rev Genet. 2005;39:503-36 PubMed
Nat Cell Biol. 1999 Sep;1(5):298-304 PubMed
Microbiology (Reading). 2001 Sep;147(Pt 9):2409-2415 PubMed
J Cell Sci. 1999 Nov;112 ( Pt 22):4151-61 PubMed
Methods Cell Biol. 2001;65:159-73 PubMed
Curr Genet. 1993 Jul-Aug;24(1-2):141-8 PubMed
