We studied the effect of increased inorganic chloride concentration on forest soil microflora in a laboratory experiment. Microbial DNA extracted from experimental soil samples was amplified with PCR using primer pairs specifically amplifying bacterial, eukaryotic and fungal DNA fragments. The resulting amplified DNA was further used for terminal restriction fragment length polymorphism (TRFLP) analysis. Our work revealed that chloride concentration affects the indigenous microbial community in experimental soil. This was documented on an unidentified microorganism whose DNA was detectable in soil high in chloride but was not found in soil with low chloride concentration. The presence of the organism responsive to increased chloride concentration was associated with the highest observed value of chlorination of humic acid, suggesting possible role of this organism in soil chlorine turnover. High chloride concentration in the soil tended to decrease the rate of degradation of trichloroacetic acid. The problems connected with measurement of chlorination rates in soil are discussed.

Institute of Microbiology Academy of Sciences of the Czech Republic v v i Vídenská 1083 CZ14220 Prague Czech Republic

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Terminal restriction fragment length polymorphism analysis of soil microbial communities reveals interaction of fungi and chlorine bound in organic matter

Local distribution of ectomycorrhizae-associated basidiomycetes in forest soil correlates with the degree of soil organic matter humification and available electrolytes