Acute and delayed implantation of positively charged 2-hydroxyethyl methacrylate scaffolds in spinal cord injury in the rat
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18173349
DOI
10.3171/spi-08/01/067
Knihovny.cz E-resources
- MeSH
- Axons pathology physiology MeSH
- Biocompatible Materials chemistry therapeutic use MeSH
- Time Factors MeSH
- Cysts pathology MeSH
- Wound Healing physiology MeSH
- Hydrogels chemistry therapeutic use MeSH
- Rats MeSH
- Methacrylates chemistry therapeutic use MeSH
- Spinal Cord blood supply pathology MeSH
- Disease Models, Animal MeSH
- Neurofibrils ultrastructure MeSH
- Paraplegia physiopathology MeSH
- Connective Tissue pathology MeSH
- Spinal Cord Injuries surgery MeSH
- Rats, Wistar MeSH
- Nerve Regeneration physiology MeSH
- Guided Tissue Regeneration MeSH
- Schwann Cells pathology MeSH
- Tissue Scaffolds * MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- 2-(trimethylammonio)ethyl methacrylate MeSH Browser
- Biocompatible Materials MeSH
- Hydrogels MeSH
- hydroxyethyl methacrylate MeSH Browser
- Methacrylates MeSH
OBJECT: Hydrogels are nontoxic, chemically inert synthetic polymers with a high water content and large surface area that provide mechanical support for cells and axons when implanted into spinal cord tissue. METHODS: Macroporous hydrogels based on 2-hydroxyethyl methacrylate (HEMA) were prepared by radical copolymerization of monomers in the presence of fractionated NaCl particles. Male Wistar rats underwent complete spinal cord transection at the T-9 level. To bridge the lesion, positively charged HEMA hydrogels were implanted either immediately or 1 week after spinal cord transection; control animals were left untreated. Histological evaluation was performed 3 months after spinal cord transection to measure the volume of the pseudocyst cavities and the ingrowth of tissue elements into the hydrogels. RESULTS: The hydrogel implants adhered well to the spinal cord tissue. Histological evaluation showed ingrowth of connective tissue elements, blood vessels, neurofilaments, and Schwann cells into the hydrogels. Morphometric analysis of lesions showed a statistically significant reduction in pseudocyst volume in the treated animals compared with controls and in the delayed treatment group compared with the immediate treatment group (p < 0.001 and p < 0.05, respectively). CONCLUSIONS: Positively charged HEMA hydrogels can bridge a posttraumatic spinal cord cavity and provide a scaffold for the ingrowth of regenerating axons. The results indicate that delayed implantation can be more effective than immediate reconstructive surgery.
References provided by Crossref.org
Aligned hydrogel tubes guide regeneration following spinal cord injury
Modified Methacrylate Hydrogels Improve Tissue Repair after Spinal Cord Injury
