Acute and delayed implantation of positively charged 2-hydroxyethyl methacrylate scaffolds in spinal cord injury in the rat
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
18173349
DOI
10.3171/spi-08/01/067
Knihovny.cz E-zdroje
- MeSH
- axony patologie fyziologie MeSH
- biokompatibilní materiály chemie terapeutické užití MeSH
- časové faktory MeSH
- cysty patologie MeSH
- hojení ran fyziologie MeSH
- hydrogely chemie terapeutické užití MeSH
- krysa rodu Rattus MeSH
- methakryláty chemie terapeutické užití MeSH
- mícha krevní zásobení patologie MeSH
- modely nemocí na zvířatech MeSH
- neurofibrily ultrastruktura MeSH
- paraplegie patofyziologie MeSH
- pojivová tkáň patologie MeSH
- poranění míchy chirurgie MeSH
- potkani Wistar MeSH
- regenerace nervu fyziologie MeSH
- řízená tkáňová regenerace MeSH
- Schwannovy buňky patologie MeSH
- tkáňové podpůrné struktury * MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- 2-(trimethylammonio)ethyl methacrylate MeSH Prohlížeč
- biokompatibilní materiály MeSH
- hydrogely MeSH
- hydroxyethyl methacrylate MeSH Prohlížeč
- methakryláty MeSH
OBJECT: Hydrogels are nontoxic, chemically inert synthetic polymers with a high water content and large surface area that provide mechanical support for cells and axons when implanted into spinal cord tissue. METHODS: Macroporous hydrogels based on 2-hydroxyethyl methacrylate (HEMA) were prepared by radical copolymerization of monomers in the presence of fractionated NaCl particles. Male Wistar rats underwent complete spinal cord transection at the T-9 level. To bridge the lesion, positively charged HEMA hydrogels were implanted either immediately or 1 week after spinal cord transection; control animals were left untreated. Histological evaluation was performed 3 months after spinal cord transection to measure the volume of the pseudocyst cavities and the ingrowth of tissue elements into the hydrogels. RESULTS: The hydrogel implants adhered well to the spinal cord tissue. Histological evaluation showed ingrowth of connective tissue elements, blood vessels, neurofilaments, and Schwann cells into the hydrogels. Morphometric analysis of lesions showed a statistically significant reduction in pseudocyst volume in the treated animals compared with controls and in the delayed treatment group compared with the immediate treatment group (p < 0.001 and p < 0.05, respectively). CONCLUSIONS: Positively charged HEMA hydrogels can bridge a posttraumatic spinal cord cavity and provide a scaffold for the ingrowth of regenerating axons. The results indicate that delayed implantation can be more effective than immediate reconstructive surgery.
Citace poskytuje Crossref.org
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