Spinal cord injury (SCI) results in neural loss and consequently motor and sensory impairment below the injury. There are currently no effective therapies for the treatment of traumatic SCI in humans. Various animal models have been developed to mimic human SCI. Widely used animal models of SCI are complete or partial transection or experimental contusion and compression, with both bearing controversy as to which one more appropriately reproduces the human SCI functional consequences. Here we present in details the widely used procedure of complete spinal cord transection as a faithful animal model to investigate neural and functional repair of the damaged tissue by exogenous human transplanted cells. This injury model offers the advantage of complete damage to a spinal cord at a defined place and time, is relatively simple to standardize and is highly reproducible.

] Spebo Medical Leskovac Serbia [2] Human Genetics Department Faculty of Medical Sciences University of Kragujevac Serbia

] Stem Cell therapies in Neurodegenerative diseases Lab Research Center Principe Felipe c Eduardo Primo Yúfera 3 46012 Valencia Spain [2] Institute of Experimental Medicine Department of Neuroscience Academy of Science of the Czech Republic Prague Czech Republic

Institute of Experimental Medicine Department of Neuroscience Academy of Science of the Czech Republic Prague Czech Republic

Neuronal and Tissue Regeneration Lab Centro de Investigación Príncipe Felipe Valencia Spain

Stem Cell therapies in Neurodegenerative diseases Lab Research Center Principe Felipe c Eduardo Primo Yúfera 3 46012 Valencia Spain

Translational Research in Fetal Surgery for Congenital Malformations Center for Fetal Cellular and Molecular Therapy Cincinnati Children's Hospital Medical Center

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Stem Cells and Labeling for Spinal Cord Injury