Properties of mixed cationic membranes studied by fluorescence solvent relaxation
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- 2-naftylamin analogy a deriváty chemie MeSH
- dimyristoylfosfatidylcholin chemie MeSH
- fluorescence MeSH
- fluorescenční barviva chemie MeSH
- kationty chemie MeSH
- kvartérní amoniové sloučeniny chemie MeSH
- laurany chemie MeSH
- lipidové dvojvrstvy chemie MeSH
- lipidy chemie MeSH
- membrány chemie MeSH
- molekulární struktura MeSH
- myristáty chemie MeSH
- rozpouštědla chemie MeSH
- voda chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,2-dimyristoyl-3-trimethylammonium propane MeSH Prohlížeč
- 2-naftylamin MeSH
- dimyristoylfosfatidylcholin MeSH
- fluorescenční barviva MeSH
- kationty MeSH
- kvartérní amoniové sloučeniny MeSH
- laurany MeSH
- laurdan MeSH Prohlížeč
- lipidové dvojvrstvy MeSH
- lipidy MeSH
- myristáty MeSH
- rozpouštědla MeSH
- voda MeSH
The need for detailed biophysical description of cationic lipid membranes, which are commonly used as gene transfection vectors, led us to study the properties of mixed cationic/zwitterionic lipid bilayers. Fluorescence solvent relaxation measurements of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) incorporated in a membrane consisting of cationic dimyristoyltrimethylammoniumpropane (DMTAP) and zwitterionic dimyristoylphosphatidylcholine (DMPC) were performed. The obtained results are compared with a recently measured system consisting of dioleoyltrimethylammoniumpropane (DOTAP) and dioleoylphosphatidylcholine (DOPC) (Jurkiewicz et al. Langmuir 22:8741-8749, 2006). The similar nonmonotonic dependence of the relaxation kinetics on cationic lipid content in the membrane was present for both systems. While the slowest solvent relaxation have been observed for 30 mol% of DOTAP in the DOPC bilayer (Jurkiewicz et al. Langmuir 22:8741-8749, 2006), for DMPC/DMTAP system it was found at 45 mol% of DMTAP, which agrees with the literature. Both membranes increased their hydration upon increased cationic lipid content.
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