Properties of mixed cationic membranes studied by fluorescence solvent relaxation
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- 2-Naphthylamine analogs & derivatives chemistry MeSH
- Dimyristoylphosphatidylcholine chemistry MeSH
- Fluorescence MeSH
- Fluorescent Dyes chemistry MeSH
- Cations chemistry MeSH
- Quaternary Ammonium Compounds chemistry MeSH
- Laurates chemistry MeSH
- Lipid Bilayers chemistry MeSH
- Lipids chemistry MeSH
- Membranes chemistry MeSH
- Molecular Structure MeSH
- Myristates chemistry MeSH
- Solvents chemistry MeSH
- Water chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-dimyristoyl-3-trimethylammonium propane MeSH Browser
- 2-Naphthylamine MeSH
- Dimyristoylphosphatidylcholine MeSH
- Fluorescent Dyes MeSH
- Cations MeSH
- Quaternary Ammonium Compounds MeSH
- Laurates MeSH
- laurdan MeSH Browser
- Lipid Bilayers MeSH
- Lipids MeSH
- Myristates MeSH
- Solvents MeSH
- Water MeSH
The need for detailed biophysical description of cationic lipid membranes, which are commonly used as gene transfection vectors, led us to study the properties of mixed cationic/zwitterionic lipid bilayers. Fluorescence solvent relaxation measurements of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) incorporated in a membrane consisting of cationic dimyristoyltrimethylammoniumpropane (DMTAP) and zwitterionic dimyristoylphosphatidylcholine (DMPC) were performed. The obtained results are compared with a recently measured system consisting of dioleoyltrimethylammoniumpropane (DOTAP) and dioleoylphosphatidylcholine (DOPC) (Jurkiewicz et al. Langmuir 22:8741-8749, 2006). The similar nonmonotonic dependence of the relaxation kinetics on cationic lipid content in the membrane was present for both systems. While the slowest solvent relaxation have been observed for 30 mol% of DOTAP in the DOPC bilayer (Jurkiewicz et al. Langmuir 22:8741-8749, 2006), for DMPC/DMTAP system it was found at 45 mol% of DMTAP, which agrees with the literature. Both membranes increased their hydration upon increased cationic lipid content.
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