SsrA genes of streptomycetes and association of proteins to the tmRNA during development and cellular differentiation
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- RNA, Bacterial genetics metabolism MeSH
- Cell Differentiation physiology MeSH
- RNA, Messenger metabolism MeSH
- Molecular Sequence Data MeSH
- RNA-Binding Proteins metabolism MeSH
- Protein Biosynthesis MeSH
- Electrophoretic Mobility Shift Assay MeSH
- Ribonucleoproteins metabolism MeSH
- Ribosomal Proteins metabolism MeSH
- RNA, Transfer metabolism MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Spores, Bacterial metabolism MeSH
- Streptomyces coelicolor genetics growth & development MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Bacterial MeSH
- RNA, Messenger MeSH
- RNA-Binding Proteins MeSH
- Ribonucleoproteins MeSH
- Ribosomal Proteins MeSH
- RNA, Transfer MeSH
- small protein B MeSH Browser
- tmRNA MeSH Browser
Transfer-messenger RNA (tmRNA, 10Sa RNA, ssrA) is bacterial RNA having both tRNA and mRNA properties and playing an essential role in recycling of 70S ribosomes that are stalled on defective mRNA. The trans-translational system is thought to play a crucial role in bacterial survival under adverse conditions. Streptomycetes are Gram-positive soil bacteria exposed to various physical and chemical stresses that activate specialized responses such as synthesis of antibiotics and morphological differentiation. Comparative sequence analysis of ssrA genes of streptomycetes revealed the most significant differences in the central parts of tag-reading frames, in the stop codons and in the 15-34 nucleotide sequences following stop codons. A major challenge in understanding the interactions that control the function of tmRNA is the definition of protein interactions. Proteins from various phases of development of Streptomyces aureofaciens associated with tmRNA were analyzed. Using affinity chromatography on tmRNA-Sepharose and photo cross-linking experiments with [(32)P]labeled tmRNA seven proteins, the beta and beta'-subunits of DNA dependent RNA polymerase, polyribonucleotide nucleotidyltransferase (PNPase), ribosomal protein SS1, ATP-binding cassette transporters, elongation factor Tu, and SmpB were identified among the proteins associated with tmRNA of S. aureofaciens. We examined the functional role of ribosomal protein SS1 in a defined in vitro trans-translation system. Our data show that the protein SS1 that structurally differs from S1 of Escherichia coli is required for translation of the tmRNA tag-reading frame.
References provided by Crossref.org
6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor
Secondary Metabolites Produced during the Germination of Streptomyces coelicolor
A Waking Review: Old and Novel Insights into the Spore Germination in Streptomyces
General and molecular microbiology and microbial genetics in the IM CAS
Biocomputational prediction of small non-coding RNAs in Streptomyces
