Differentiation of CD133-positive pancreatic cells into insulin-producing islet-like cell clusters
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18374086
DOI
10.1016/j.transproceed.2008.02.017
PII: S0041-1345(08)00126-7
Knihovny.cz E-resources
- MeSH
- AC133 Antigen MeSH
- Cell Differentiation MeSH
- Cell Culture Techniques MeSH
- C-Peptide analysis MeSH
- Antigens, CD analysis MeSH
- Glycoproteins analysis MeSH
- Islets of Langerhans cytology physiology MeSH
- Humans MeSH
- Magnetics MeSH
- Pancreas cytology MeSH
- Peptides analysis MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- RNA genetics isolation & purification MeSH
- Cell Separation methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- AC133 Antigen MeSH
- C-Peptide MeSH
- Antigens, CD MeSH
- Glycoproteins MeSH
- Peptides MeSH
- PROM1 protein, human MeSH Browser
- RNA MeSH
Adult pancreatic stem and progenitor cells could represent an alternative source of insulin-producing tissue for diabetes treatment. In order to identify these cells, we have focused on the human pancreatic cells expressing cell surface molecule CD133, a marker of adult stem cells. We found that population of human CD133-positive pancreatic cells contains endocrine progenitors expressing neurogenin-3 and cells expressing human telomerase, ABCG2, Oct-3/4, Nanog, and Rex-1, markers of pluripotent stem cells. These cells were able to differentiate into insulin-producing cells in vitro and secreted C-peptide in a glucose-dependent manner. Based on our results, we suppose that the CD133 molecule represents another cell surface marker suitable for identification and isolation of pancreatic endocrine progenitors.
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